A STUDY OF STRESS PROTEIN IN HUMAN SKELETAL MUSCLE

人类骨骼肌中应激蛋白的研究

• 批准号: 16300212
• 负责人: NAITO Hisashi
• 金额: $ 8.22万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300212/
• 关键词: Stress proteins; Heat shock protein 72; Muscle biopsy; Microwave hyperthermia; Skeletal muscle; Exercise; Delayed onset muscle soreness; ヒートショックタンパク質; 繰り返し効果; 高齢者; ハイパーサーミア; 筋温; 国際情報交換; アメリ力合衆国:トルコ共和国; HSP70; 外側広筋; アメリカ合衆国:トルコ共和国; ミオシン重鎖; Stress proteins; Heat shock protein 72; Muscle biopsy; Microwave hyperthermia; Skeletal muscle; Exercise; Delayed onset muscle soreness; ヒートショックタンパク質; 繰り返し効果; 高齢者; ハイパーサーミア; 筋温; 国際情報交換; アメリ力合衆国:トルコ共和国; HSP70; 外側広筋; アメリカ合衆国:トルコ共和国; ミオシン重鎖

It was unclear that the responses of stress protein expression to the exercise and the heat-stress in human skeletal muscles. In this study, we indicated that safe and reliable modality for inducing stress proteins in human skeletal muscle. Moreover, we also investigated the possibility of applying our results to clinical training fields. The outcome of this study was shown as follows:(1) We established the approach to research stress proteins in human skeletal muscle by using newly improved needle biopsy technique which did not disturb daily activity and training of subjects Therefore, we could develop the research field with human muscle biopsy technique.(2) It is well known that there is individual variation of stress protein expression level in sedentary condition. We revealed the stress protein expression level correlated with muscle fiber composition and strongly expressed in endurance athlete. Moreover, stress protein showed less expression level in a paralysis patient with muscle atrophy.(3) We indicated that local microwave hyperthermia treatment can increase stress proteins in human skeletal muscle. Moreover, we worked on development of the microwave hyperthermia system to provide safe and effective method to induce stress proteins in human skeletal muscle. Our method maintained the skin temperature around 40℃ and increased muscle temperature until 45℃ without muscle damage.(4) The muscle preconditioning with a microwave hyperthermia system may prevent eccentric exercise-induced muscle damage, although the effect may not apply to repeated bouts of eccentric exercise.Further investigation should examine time course of stress proteins induced by heat stress, and effect on performance in athlete.

目前尚不清楚应激蛋白表达对运动的反应以及人类骨骼肌中的热压力。在这项研究中，我们指出，人类骨骼肌中诱导的应激蛋白的安全可靠方式。此外，我们还研究了将结果应用于临床培训领域的可能性。这项研究的结果如下：（1）我们通过使用新的改进的针头活检技术来确定人类骨骼肌中的应激蛋白的方法，该技术不会干扰日常活动和对受试者的培训，因此我们可以通过人类肌肉活检技术开发研究领域。我们揭示了应力蛋白表达水平与肌肉纤维组成相关，并且在耐力运动员中强烈表达。此外，在肌肉萎缩的麻痹患者中，应激蛋白表现出较少的表达水平。（3）我们指出，局部微波高温治疗可以增加人类骨骼肌中的应激蛋白。此外，我们研究了微波高温系统的开发，以提供安全有效的方法来诱导人骨骼肌中的应激蛋白。我们的方法将皮肤温度保持在40℃左右，并升高肌肉温度，直到45℃而不会造成肌肉损伤。（4）通过微波高温系统的肌肉预处理可能会阻止偏心运动引起的肌肉损害，尽管该效果可能不适用于偏心运动的反复出现。FURURY应检查由热压力诱导的压力蛋白的效果，并在运动中效应，并在运动中效应，并在运动中效应。