A STUDY OF STRESS PROTEIN IN HUMAN SKELETAL MUSCLE

人类骨骼肌中应激蛋白的研究

基本信息

批准号：
16300212
负责人：
NAITO Hisashi
金额：
$ 8.22万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

It was unclear that the responses of stress protein expression to the exercise and the heat-stress in human skeletal muscles. In this study, we indicated that safe and reliable modality for inducing stress proteins in human skeletal muscle. Moreover, we also investigated the possibility of applying our results to clinical training fields. The outcome of this study was shown as follows:(1) We established the approach to research stress proteins in human skeletal muscle by using newly improved needle biopsy technique which did not disturb daily activity and training of subjects Therefore, we could develop the research field with human muscle biopsy technique.(2) It is well known that there is individual variation of stress protein expression level in sedentary condition. We revealed the stress protein expression level correlated with muscle fiber composition and strongly expressed in endurance athlete. Moreover, stress protein showed less expression level in a paralysis patient with muscle atrophy.(3) We indicated that local microwave hyperthermia treatment can increase stress proteins in human skeletal muscle. Moreover, we worked on development of the microwave hyperthermia system to provide safe and effective method to induce stress proteins in human skeletal muscle. Our method maintained the skin temperature around 40℃ and increased muscle temperature until 45℃ without muscle damage.(4) The muscle preconditioning with a microwave hyperthermia system may prevent eccentric exercise-induced muscle damage, although the effect may not apply to repeated bouts of eccentric exercise.Further investigation should examine time course of stress proteins induced by heat stress, and effect on performance in athlete.

目前尚不清楚人体骨骼肌中应激蛋白表达对运动和热应激的反应。此外，我们还研究了这种可能性。将我们的结果应用到临床训练领域的结果如下：（1）我们建立了通过使用新改进的针吸活检来研究人类骨骼肌中的应激蛋白的方法，该技术不干扰日常活动和训练。因此，我们(2)众所周知，久坐状态下应激蛋白表达水平存在个体差异，我们揭示了应激蛋白表达水平与肌纤维组成相关，并在耐力运动员中强烈表达。此外，在肌肉萎缩的瘫痪患者中，应激蛋白的表达水平较低。(3)我们表明，局部微波热疗可以增加人体骨骼肌中的应激蛋白。此外，我们致力于微波热疗系统的开发，以提供安全的治疗。以及有效的方法我们的方法将皮肤温度维持在40℃左右，并将肌肉温度提高到45℃，而不会造成肌肉损伤。（4）用微波热疗系统进行肌肉预处理可以防止离心运动引起的肌肉损伤，尽管效果可能不适用于重复的离心运动。进一步的研究应该检查热应激引起的应激蛋白的时间过程，以及对运动员表现的影响。