Survey of estrogenic contamination in the costal waters of East China Sea using grey mullet.

东海近海灰鲻鱼雌激素污染调查.

基本信息

批准号：
15405005
负责人：
SOYANO Kiyoshi
金额：
$ 7.81万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405005/
关键词：
Endocrine disruption grey mullet abnormality vitellogenin East China Sea

项目摘要

It is well known that endocrine and sexual disruptions in wild teleosts are induced by endocrine disrupting chemicals which have an estrogen-like function. However, the actualities of contamination by these substances in aquatic environment of coastal waters are not investigated well in Japan and East Asia. We carried out the monitoring of environmental estrogens in marine and estuarine areas using wild grey mullet (Mugil cephalus). In this research, we tried to determine the plasma concentrations of vitellogenin (VTG) which is the major yolk protein produced by the liver under the influence of estrogen. Moreover, we observed gonadal abnormalities using histological technique.VTG levels of grey mullets in the bay areas were significantly higher than the ones of fish collected in the open sea. In male and immature female fish of Tokyo Bay, Osaka Bay, Japan, VTG levels were the same as mature female fish. These areas are fed by a large amount of industrial and domestic sewage and effluent. VTG level is generally very low (<1μg/ml) in male and immature fish, because vitellogenic oocytes are not included in the gonad of these fish. These results indicate that VTG synthesis was induced by exogenous estrogen-like substance (environmental estrogen). VTG levels of the fish collected from South part of Korea were higher than another place of Korea.We will start the new survey of estrogenic contaminations in costal waters in the East China Sea with scientist of Korea and China. Moreover, we have a plan to construct the network of scientist for the research of endocrine disruption in East Asia.

众所周知，野生硬骨料中的内分泌和性破坏是通过内分泌破坏具有雌激素样功能的化学物质引起的。但是，在日本和东亚，这些物质在沿海水域的水生环境中受到污染的现实尚未得到很好的研究。我们使用野生灰m鱼（Mugil Cephalus）对海洋和河口区域的环境雌激素进行监测。在这项研究中，我们试图确定果胶（VTG）的血浆浓度，这是肝脏在雌激素影响下产生的主要蛋白蛋白。此外，我们使用组织学技术观察到性腺异常。海湾地区的VTG水平高于在开海中收集的鱼类。在日本大阪湾东京湾的雄性和不成熟的雌鱼中，VTG水平与成熟的雌鱼相同。这些区域VTG水平由大量工业和家庭污水供应。雄性和未成熟鱼的VTG水平通常非常低（<1μg/ml），因为这些鱼的性腺中不包括卵黄细胞。这些结果表明，VTG合成是由外源性雌激素样物质（环境雌激素）诱导的。从韩国南部收集的鱼类水平高于韩国的另一个地方。我们将与韩国和中国的科学家开始对东中国哥斯多海水域中雌激素污染的新调查。此外，我们计划构建科学家网络，以研究东亚内分泌干扰的研究。