Systematic survery for biologically active peptides in the atrium and pheochromocytoma tissue.

系统调查心房和嗜铬细胞瘤组织中的生物活性肽。

基本信息

批准号：
15390251
负责人：
KITAMURA Kazuo
金额：
$ 9.47万
依托单位：
University of Miyazaki (2004-2005)宮崎医科大学 (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

We discovered adrenomedullin (AM) in 1993 and found another hypetensive peptide proadrenomedullin N-terminal 20 peptide (PAMP) which is produced from adrenomedullin precursor. We have clarified that AM is a new cardiovascular hormone to regulate circulation and body fluid homeostasis. Further, AM shows protective actions for the heart and blood vessels in vitro and in vivo, suggesting that AM may be a new drugs for cardiovascular and inflammatory diseases. Thus, it is important to discover the new hormone and to study its function. We have concurrently advanced the another project to discover still unidentified vasoactive peptide in the porcine atrium and human pheochromocytoma tissue.AM was discovered monitoring the activity that elevate cAMP in the rat platelet. We have searched biological active peptide using the cAMP elevation in cultured cells from pheochromocytoma. Using this assay, we isolated several peptides from pheochromocytoma tissue. These peptide were found to be VIP, PAC … More AP, CGRP and PHM. These peptides were known peptides, but we have isolated novel peptide which exist in the the pheochromocytoma. Many biologically active peptides has C-terminal amide structure which is essential for biological activity. We have established a novel method to detect a peptide with C-terminal amide structure. Using this method, we have isolated a novel 14 amino acids peptide in the atrium whose C-terminus was amidated. This peptide was termed amidicin. Amidicin is widely distributed in porcine tissue and is especially abundant in pituitary gland, cardiac ventricle and spleen. We have developed radioimmunoassay for human amidicin, and examined the distribution of amidicin in human tissue. We found that human amidicin is abundant in human pheochromocytoma tissue. We purified human amidicin from pheochromocytoma and determined its structure. Furthermore, amidicin was found to circulate in human blood in a considerable concentration. Amidicin may be a possible new peptide participating in circulation control. Less

我们在1993年发现了肾上腺素素（AM），而创始人的hypetide肽培训蛋白N-末端20肽（Pamptide）Drenomed Urlin前体我们已经澄清了AM IS IS IS IS是一种新的心血管激素，以调节和身体流体液体固定剂对于体外和体内的theart骨血管，可能会出现托里疾病，因此，发现新的激素并研究其功能，以发现猪心房和人类植物瘤组织中的其他血管活性肽。大鼠血小板中的活动是使用培养细胞中的cAMP升高的生物活性肽。但是，我们的新型肽存在于理性细胞瘤中。 C-末端被称为肌动蛋白。。。较少的

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of C-reactive protein on atherogenic mediators and adrenomedullin in human coronary artery endothelial and smooth muscle cells.

C反应蛋白对人冠状动脉内皮细胞和平滑肌细胞中致动脉粥样硬化介质和肾上腺髓质素的影响。

DOI：
发表时间：
2004
期刊：
Biochem Biophys Res Commun. 314
影响因子：
0
作者：
Nagoshi;Y.;Kuwasako;K.;Cao;Y.N.;Kitamura;K.;Eto;T.
通讯作者：
T.

Adrenomedullin alleviates not only neointimal formation but also perivascular hyperplasia following arterial injury in rats.

肾上腺髓质素不仅可以减轻大鼠动脉损伤后的新内膜形成，还可以减轻血管周围增生。

DOI：
发表时间：
2005
期刊：
European Journal of Pharmacology 508(1-3)
影响因子：
0
作者：
Tsuruda T;Kato J;Matsui E;Hatakeyama K;Masuyama H;Imamura T;Kitamura K;Asada Y;Eto T
通讯作者：
Eto T

Mishima K, et al.: "Angiotensin II modulates gene expression of adrenomedullin receptor components in rat cardiomyocytes"Life Sci. 73. 1629-1635 (2003)

Mishima K 等人：“血管紧张素 II 调节大鼠心肌细胞中肾上腺髓质素受体成分的基因表达”Life Sci。