Genetic analysis of measles viruses isolated in Japan and mechanism of transmission-role of antibodies induced by vaccination

日本分离的麻疹病毒基因分析及传播机制——疫苗接种诱导抗体的作用

基本信息

批准号：
15390211
负责人：
ITOH Masae
金额：
$ 8.45万
依托单位：
Nagahama Institute of Bio-Science and Technology (2004-2006)Osaka Prefectural Institute of Public Health (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390211/
关键词：
measles virus vaccine antibody genome analysis H gene N gene cell fusion diversity 多核巨細胞 F遺伝子

项目摘要

Nucleotide sequences of the H and N genes of measles viruses isolated in Japan since 1980s were determined and compared with those obtained from databases. By examining accumulation of the mutations and genetic development of the genes, the origin of each virus was assumed and the affect of antibodies acquired by vaccination on the rate of accumulation of the mutations was discussed.Type D3 virus : Type D3 viruses were prevailing in the late 1980s and the early 1990s, which were replaced by Type D5 viruses and emerged again in 1997. Re-emerging D3 viruses originated from the Guam.USA/94 like strain and mutated into two lineages in Japan.Type D5 virus : Type D5 viruses in the early 2000s were supposed to be the progenitor of type D5 viruses in the early 1990s and the most probable ancestor was the Palau.BLA/93 like virus. In 2007, the measles virus infection burst around Tokyo and is now spreading all over Japan. The isolated viruses from the epidemic belong to the different lineage of type D5 from that of type D5 viruses in the early 2000s, to the same lineage with MVi/Queensland.AU/37.03.Type H1 virus : Type H1 viruses have been isolated in Japan since 1999. Type H1 viruses isolated in Japan could be divided into two groups and there is a possibility that two kinds of type H1 virus possessing different gene sequences invaded into Japan.Isolates from vaccinated patients : H and N genes of the viruses from the vaccinated patients were compared with those of isolates from the same epidemic. We could not confirm the increased accumulation of mutations with the viruses isolated from vaccinated patients.Diversity of measles virus : A strain of measles virus derived from a patient revealed not to be genetically single but to be a mixed population.

自1980年代以来在日本分离的麻疹病毒的H和N基因的核苷酸序列被确定，并与从数据库中获得的核苷酸序列进行了比较。通过检查基因突变和遗传发育的积累，讨论了每种病毒的起源，并讨论了疫苗接种获得的抗体的影响。讨论了1980年代和1990年代末和1990年代初，D3病毒在1980年代末和D5 Viruse替换为D5 Vireers and Emerem and emeremed and emeremed and emeremed and emeremed and emeremed and emeremed and emeremed and emered and emered and ersemed and emere emered and emered and emered reperem reperem proped type d3病毒：类型D3病毒。 Guam.usa/94像菌株一样，在日本进行突变成两个谱系。D5病毒：2000年代初期的D5病毒被认为是1990年代初期D5病毒的祖细胞，最可能的祖先是Palau.bla.bla/93，如病毒。 2007年，麻疹病毒感染在东京周围爆发，现在正在遍布日本。来自该流行病的孤立病毒属于2000年代初与D5型病毒的不同谱系，与MVI/Queensland.AU/37.03.Type H1病毒：H1型病毒在日本自1999年以来孤立的H1病毒在日本中孤立的H1病毒在日本上孤立的，与H1型相同的H1病毒可能是孤立的。从接种疫苗的患者中渗透到日本的基因序列：接种疫苗患者的病毒的H和N基因与同一流行病的分离株的患者进行了比较。我们无法确认从接种疫苗的患者中分离出的病毒的突变积累增加。麻疹病毒的多样性：从患者中得出的麻疹病毒菌株表明不是遗传性单一，而是混合种群。