The establishment of the experimental animal model of joint contracture, and analysis of the marker gene

关节挛缩实验动物模型的建立及标记基因分析

基本信息

批准号：
15300195
负责人：
ETO Fumio
金额：
$ 10.24万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

This study was to examine a change of joint and muscle in the experimental model in order to elucidate the pathology of joint contracture as a symptom of disuse syndromes. We made experimental animal model of joint contracture. Neurotomy of the left sciatic nerve was performed on the male 9-week-old Wister rats of 9 weeks old. The right side as a control was not treated. A calf muscle of rat developed a remarkable atrophy at 30th day after neurotomy, and the knee joint showed a flexion position with decreased range of motion. With articular cartilage of the prepared animal model of joint contracture we evaluated the known gene of type II collagen and aggrecan by the quantitative RT-PCR. There was no significant difference in the estimated type II collagen gene expression level between the denervated group and control group. The estimated gene expression level of aggrecan revealed a significant difference between the two groups. Then we examined the number of chondrocyte and the change … More of thickness of a cartilage layer. The average number of chondrocyte did not show any tendency of increase nor tendency of decrease between the two groups during the experimental period. As for the thickness of articular cartilage, the denervated group showed thicker than the control group except for 7th and 14th day after neurotomy. The number of chondrocyte and configuration of the cartilage layer revealed little changes after neurotomy. In addition, we investigated quantitative evaluation of muscle atrophy of denervated rat using MRI. We tried to estimate the muscle diameter and diffusion coefficient inside the muscle with using the water and creatine spectral analysis. There was no significant difference in the estimated diffusion coefficient between the denervated and control groups. The estimated fiber diameter of denervated group showed smaller compared with that of the the control group at the 8th week after neurotomy. This result agreed with the fact that the neurotomy caused muscle atrophy, but the difference was not statistically significant. In denervated group, the creatine content of the gastrocnemius muscle per unit volume (voxel) was smaller than that of the control group. We could not detect the unknown gene which was predicted in the articular cartilage of produced neurogenic joint contracture. This study revealed the complexity of pathophysiology in the development of the joint contracture with having evaluated comprehensively the tissues involved in joint contracture including a search of marker gene. Less

本研究旨在检查实验模型中关节和肌肉的变化，以阐明作为废用综合征症状的关节挛缩的病理学，我们对左侧坐骨神经进行了神经切断术的实验动物模型。 9周龄雄性Wister大鼠，右侧作为对照，神经切断后第30天，小腿肌肉出现明显萎缩，膝关节出现明显萎缩。对于所制备的关节挛缩动物模型的关节软骨，我们通过定量RT-PCR评估了已知的II型胶原蛋白和聚集蛋白聚糖基因，估计的II型胶原蛋白基因表达没有显着差异。去神经组和对照组之间的聚集蛋白聚糖的估计基因表达水平显示出两组之间的显着差异。然后我们检查了软骨细胞的数量和软骨层的平均数量的变化。实验期间，两组间软骨细胞的数量均未出现增加或减少的趋势，至于关节软骨的厚度，除神经切断后第7天和第14天外，去神经组的软骨细胞数量均较对照组厚。神经切断后软骨细胞的结构和软骨层的结构几乎没有变化。此外，我们研究了使用 MRI 对去神经大鼠肌肉萎缩的定量评估。使用水和肌酸光谱分析估计的肌肉内部纤维直径和扩散系数，去神经组和对照组之间的估计纤维直径没有显着差异。神经切断术后第8周，这一结果与神经切断导致肌肉萎缩的事实相符，但去神经组中腓肠肌的肌酸含量差异无统计学意义。我们无法检测到在产生的神经源性关节挛缩的关节软骨中预测的未知基因。这项研究揭示了关节挛缩发展的病理生理学的复杂性。全面评估了参与关节结构的组织，包括搜索标记基因。

项目成果

期刊论文数量（47）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Magnetic resonance imaging of electrical conductivity in the human brain

DOI：
10.1109/tmag.2009.2016418
发表时间：
2005
期刊：
IEEE Transactions on Magnetics
影响因子：
2.1
作者：
M. Sekino;Yusuke Inoue;S. Ueno
通讯作者：
M. Sekino;Yusuke Inoue;S. Ueno

The state of the art of nanobioscience in Japan

日本纳米生物科学的最新水平

DOI：
发表时间：
2006
期刊：
IEEE Transactions on Nanobioscience 5・1
影响因子：
0
作者：
Eguchi Y;et al.;Sachiko Yamaguchi et al.;Takako Saotome et al.;Shoogo Ueno et al.
通讯作者：
Shoogo Ueno et al.

Problem and view of aged society

老龄社会的问题与看法