The establishment of the experimental animal model of joint contracture, and analysis of the marker gene

关节挛缩实验动物模型的建立及标记基因分析

基本信息

批准号：
15300195
负责人：
ETO Fumio
金额：
$ 10.24万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

This study was to examine a change of joint and muscle in the experimental model in order to elucidate the pathology of joint contracture as a symptom of disuse syndromes. We made experimental animal model of joint contracture. Neurotomy of the left sciatic nerve was performed on the male 9-week-old Wister rats of 9 weeks old. The right side as a control was not treated. A calf muscle of rat developed a remarkable atrophy at 30th day after neurotomy, and the knee joint showed a flexion position with decreased range of motion. With articular cartilage of the prepared animal model of joint contracture we evaluated the known gene of type II collagen and aggrecan by the quantitative RT-PCR. There was no significant difference in the estimated type II collagen gene expression level between the denervated group and control group. The estimated gene expression level of aggrecan revealed a significant difference between the two groups. Then we examined the number of chondrocyte and the change … More of thickness of a cartilage layer. The average number of chondrocyte did not show any tendency of increase nor tendency of decrease between the two groups during the experimental period. As for the thickness of articular cartilage, the denervated group showed thicker than the control group except for 7th and 14th day after neurotomy. The number of chondrocyte and configuration of the cartilage layer revealed little changes after neurotomy. In addition, we investigated quantitative evaluation of muscle atrophy of denervated rat using MRI. We tried to estimate the muscle diameter and diffusion coefficient inside the muscle with using the water and creatine spectral analysis. There was no significant difference in the estimated diffusion coefficient between the denervated and control groups. The estimated fiber diameter of denervated group showed smaller compared with that of the the control group at the 8th week after neurotomy. This result agreed with the fact that the neurotomy caused muscle atrophy, but the difference was not statistically significant. In denervated group, the creatine content of the gastrocnemius muscle per unit volume (voxel) was smaller than that of the control group. We could not detect the unknown gene which was predicted in the articular cartilage of produced neurogenic joint contracture. This study revealed the complexity of pathophysiology in the development of the joint contracture with having evaluated comprehensively the tissues involved in joint contracture including a search of marker gene. Less

这项研究是检查实验模型中关节和肌肉的变化，以阐明关节收缩的病理作为废除综合征的症状。我们制作了联合收缩的实验动物模型。左坐骨神经神经的神经切开术是对9周大的9周大鼠的雄性卫生大鼠进行的。右侧作为控制没有得到处理。大鼠的小腿肌肉在神经切开术后的第30天就形成了显着的萎缩，膝关节显示出屈曲位置，运动范围有所改善。通过定量的RT-PCR，使用了共同合同准备的关节动物模型的关节软骨，评估了II型胶原蛋白和Aggrecan的已知基因。被取消的组和对照组之间估计的II型胶原基因表达水平没有显着差异。 Aggrecan的估计基因表达水平显示两组之间存在显着差异。然后，我们检查了软骨细胞的数量和变化……软骨层的厚度更多。在实验期间，两组之间的平均软骨细胞数没有显示出任何增加或减少趋势的趋势。至于关节软骨的厚度，除神经切开术后的第7天和第14天外，未经无神经的组显示出比对照组厚的。软骨细胞的数量和软骨层的构型显示神经切开术后几乎没有变化。此外，我们研究了使用MRI对肌肉肌肉萎缩的定量评估。我们试图通过使用水和创造光谱分析来估计肌肉内部肌肉直径和扩散核心。未经许可组和对照组之间的估计差异没有显着差异。与对照组相比，神经切开术后第8周的对照组比对照组的估计纤维直径显示较小。该结果同意神经切开术引起肌肉萎缩，但差异在统计学上并不显着。在被剥离的组中，每单位体积（体量）的胃肌肌肉的创造含量小于对照组的创造含量。我们无法检测到未知基因，该基因在产生的神经源性关节合同的关节软骨中得到了预测。这项研究揭示了病理生理学在关节缔合发展中的复杂性，并彻底评估了与关节缔合有关的组织，包括搜索标记基因。较少的

项目成果

期刊论文数量（47）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Magnetic resonance imaging of electrical conductivity in the human brain

DOI：
10.1109/tmag.2009.2016418
发表时间：
2005
期刊：
IEEE Transactions on Magnetics
影响因子：
2.1
作者：
M. Sekino;Yusuke Inoue;S. Ueno
通讯作者：
M. Sekino;Yusuke Inoue;S. Ueno

The state of the art of nanobioscience in Japan

日本纳米生物科学的最新水平

DOI：
发表时间：
2006
期刊：
IEEE Transactions on Nanobioscience 5・1
影响因子：
0
作者：
Eguchi Y;et al.;Sachiko Yamaguchi et al.;Takako Saotome et al.;Shoogo Ueno et al.
通讯作者：
Shoogo Ueno et al.

Problem and view of aged society

老龄社会的问题与看法