The production mechanism and role of lipoxygenase products in rat granulosa cells

大鼠颗粒细胞脂氧合酶产物的产生机制及作用

• 批准号: 03670794
• 负责人: OKAMURA Hitoshi
• 金额: $ 1.73万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670794/
• 关键词: granulosa cell; lipoxygenase products; steroidogenesis; folliculogenesis; ovulation; リポキシナ-ゼ代謝産物

Granulosa cells primed by diethylstilboestrol (DES) in vivo produced steroid hormones including progesterone, but not lipoxygenase products in in vitro culture. Granulosa cells primed by pregnant mare serum gonadotropin (PMSG) in vivo produced both steroid hormones and lipoxygenase products including hydroxyeicosatetraenoic acid (HETE) in in vitro culture. These findings suggested that there were different characteristics and potency between DES-primed granulosa cells and PMSG-primed granulosa cells.When the DES-primed granulosa cells cultured in the medium containing gonadotropins, they produced lipoxygenase products including HETE.The production of HETE was influenced by the addition of steroid hormones.The effect and role of lipoxygenase products on progesterone production were also studied in rat granulosa cells.The results demonstrated that the gonadotropin-primed granulosa cells could produced not only steroid hormones but also lipoxygenase products, and suggested an autocrine and paracrine regulation mechanism in steroidogenesis through lipoxygenase products, though further studies are needed.

体内经二乙基己烯雌酚 (DES) 引发的颗粒细胞产生包括黄体酮在内的类固醇激素，但在体外培养中不产生脂氧合酶产物。体内由孕马血清促性腺激素（PMSG）引发的颗粒细胞在体外培养中产生类固醇激素和脂氧合酶产物，包括羟基二十碳四烯酸（HETE）。这些结果表明，DES引发的颗粒细胞和PMSG引发的颗粒细胞之间存在不同的特征和效力。当DES引发的颗粒细胞在含有促性腺激素的培养基中培养时，它们产生包括HETE在内的脂氧合酶产物。HETE的产生受到影响通过添加类固醇激素。还在大鼠颗粒细胞中研究了脂氧合酶产品对黄体酮产生的影响和作用。结果表明，促性腺激素引发的颗粒细胞不仅可以产生类固醇激素，还可以产生脂氧合酶产物，并表明通过脂氧合酶产物在类固醇生成中存在自分泌和旁分泌调节机制，但还需要进一步研究。

项目成果

岡村 均: "卵巣内機能調節因子" 産婦人科の進歩. 45. 274-281 (1993)

Hitoshi Okamura：“卵巢内功能调节器”妇产科进展 45. 274-281 (1993)。

N.Tanaka, L.L.Espey, T.Kawano, and H.Okamura: "Comparison of inhibitory actions of indomethacin and epostane on ovulation in rats." Am.J.Physiol. 260. E170-E174 (1991)

N.Tanaka、L.L.Espey、T.Kawano 和 H.Okamura：“吲哚美辛和依波坦对大鼠排卵的抑制作用比较”。

岡村 均: "卵巣機能調節機構" 産婦人科治療. 64. 547-552 (1992)

冈村仁：《卵巢功能调节机制》妇产科治疗64。547-552（1992）。

岡村 均: "排卵の局所機序:図説産婦人科VIEW19 排卵機構とその障害" メジカルビュー社, 108-117 (1995)

Hitoshi Okamura：“排卵的局部机制：图解妇产科 VIEW 19 排卵机制及其疾病”Medical View Publishing，108-117（1995）