CHEMICAL AND GEOCHEMICAL STUDIES ON ORGANIC MATTER IN THE OCEAN

海洋有机物的化学和地球化学研究

基本信息

批准号：
03403008
负责人：
HANDA Nobuhiko
金额：
$ 13.06万
依托单位：
INSTITUTE FOR HYDROSPHERIC-ATMOSPHERIC SCIENCES NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03403008/
关键词：
Dissolved Organic Carbon Dissoved Organic Matter High Temperature Catalitic Oxidation Wet Oxidation Phytoplankton Bloom Dissolved Lipids Dissolved Amino Acids and Proteins Dissoved Carbohydrates 溶存有機炭素湿式酸化法黒潮東シナ海大陸棚域溶存脂質炭

项目摘要

Aims of this work are to establish analytical method of determining dissolved organic carbon in the seawater and to characterize chemical nature and structure of the organic materials which were separated by variuose kinds of combined chromatography and mass spectrometry.Sea water samples collected from the East Chine Sea including the continental shelf and shelf slope areas and Kuroshio area were analyzed for dissolved organic carbon by high temperatrure catalytic oxidation (HTCO-OC) and classical wet oxidation (WO-OC). WO-OC/HTCO-OC was characteristically different from areas to areas. High values were obtained in the Kuroshio areas, while low values in the continental areas of the East China Sea, indicating that phytoplankton bloom might be playing an important role for the production of dissolved organic matter in the surface of the ocean.To solve this problems, production of dissolved organic materials by phytoplankton was pursued in the mesocosm experiment, which was conducted in the Saanich Inlet, Canada. To the mesocosm, nutients were added to cause phytoplankton bloom, in which dissolved organic carbon concentration was pursued for several weeks together with other chemical and physical parameters. We clearly found that phytoplankton produced dissolved organic matrials consisting of lipid, amino acids and proteins and carbohydrates during the course of its bloom. These organic materials exuded by phytoplankton were fully characterized chemically by variouse analytical methiods such as combined gas chromatography and mass spectrometry, nuclear magnetic resonance and gas chromatography-combusion-isotope mass spectrometry. These data obtained are now partly already submited by several journals but most of them are now under preparation for the publication.

本工作的目的是建立测定海水中溶解有机碳的分析方法，并表征通过各种组合色谱和质谱分离的有机物质的化学性质和结构。东海海水样品采集通过高温催化氧化（HTCO-OC）和经典湿式氧化（WO-OC）分析了包括大陆架和陆架斜坡区域以及黑潮区域的溶解有机碳。 WO-OC/HTCO-OC 因地区而异。黑潮地区的值较高，而东海大陆地区的值较低，表明浮游植物水华可能对海洋表面溶解有机物的产生发挥着重要作用。在加拿大萨尼奇湾进行的中生态实验中，浮游植物生产溶解的有机物质。在中生态系统中，添加营养物质以引起浮游植物大量繁殖，其中溶解的有机碳浓度以及其他化学和物理参数被追踪了数周。我们清楚地发现，浮游植物在其繁殖过程中产生了由脂质、氨基酸、蛋白质和碳水化合物组成的溶解有机物质。通过气相色谱-质谱联用、核磁共振、气相色谱-燃烧-同位素质谱等多种分析方法对浮游植物分泌的这些有机物质进行了充分的化学表征。目前，获得的这些数据部分已由多家期刊提交，但大多数正在准备出版。