Molecular Phylogenetic Analysis of Asarum sect.Euheterotropa Based on Chloroplast trnL/F and Nuclear rDNA ITS Sequences.

基于叶绿体 trnL/F 和核 rDNA ITS 序列的细辛属真异性分子系统发育分析。

基本信息

批准号：
14540649
负责人：
SUGAWARA Takashi
金额：
$ 1.98万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540649/
关键词：
Molecular phylogeny Asarum Taxonomy Euheterotropa Biogeography Speciation Aristolochiaceae 系統地理葉緑体DNA

项目摘要

Asarum section Euheterotropa (Aristolochiaceae) is composed of five species endemic to Japan : A.asaroides distributed in northern Kyushu District, A.satsumense in southern Kyushu District, A.curvistigma in Tokai District., A.muramatsui in Izu Peninsula and A.tamaense in Kanto District. Morphologycally, these species share a broad calyx-tube, a complicated annulus and a style with stigma gradually decurrent to its abaxial side, and thereby they have assigned to the same section and regarded to be diversified in spreading to eastern district of Japan (Maekawa, 1953, 1964). However, the two disjunctive species groups appear to differ in origin. In order to clarify the phylogenetic and biogeographical relationships of the five species of section Euheterotrop we investigated molecular phylogeny, using DNA sequences from the chloroplast DNA region (trnL/F) and the internal transcribed spacer (ITS) of nuclear ribosomal DNA. Sequences of the trnL/F were found to be invariable and thus to be not useful in considering the phylogenetic relationships. In contrast, ITS-1 sequences were found to be informative phylogenetically. Two major clades (I and II) were revealed found to be informative phylogenetically. Two major clades (I and II) were species in Kyushu and the other three species in Tokai/Kanto did not form a common clade, and were clearly divided into the above two different clades, respectively. Therefore, it is clear that the five species belonging to the sect.Euheterotropa is not a monophyletic group. The results obtained from the present study do not support the Maekawa's hypothesis.

Asarum e节Euhetotropa（Aristolochiaceae）由日本特有的五种物种组成：A.asaroides分布在北北北部地区，位于九州南部的A.satsumensense，a.curvistigma，a.curvistigma，a.curvistigma，tokai District。从形态学上讲，这些物种具有宽阔的花萼管，复杂的环和具有污名的风格，逐渐使其横向横向，因此它们已分配给同一部分，并被认为是在传播到日本东部地区的多元化（Maekawa，1953年，1953年，1964年）。但是，两个分离的物种群似乎在起源上有所不同。为了阐明Euheterrop节的五种物种的系统发育和生物地理关系，我们使用叶绿体DNA区域（TRNL/F）的DNA序列研究了分子系统发育，而核核糖体DNA的内部转录间隔（ITS）。发现TRNL/F的序列是不变的，因此在考虑系统发育关系方面没有用。相反，发现ITS-1序列在系统发育上是有益的。发现两个主要的进化枝（I和II）在系统发育上是有益的。九州的两个主要进化枝（I和II）是物种，Tokai/Kanto中的其他三个物种并未形成共同的进化枝，并且显然分别分为上述两个不同的进化枝。因此，很明显，属于该地区的五个物种。Euheterotropa不是单一的群体。从本研究获得的结果不支持Maekawa的假设。