Functional analysis of plastid transcription factors by chloroplast transformation.

通过叶绿体转化对质体转录因子进行功能分析。

• 批准号: 14540598
• 负责人: SHIINA Takashi
• 金额: $ 2.43万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540598/
• 关键词: PEP; NEP; transcription; sigma factor; promoter; RNA polvmeraze; chloroplast; Arabidopsis; SIG1; SIG5; SIBI; 葉緑体形質転換; 大量発現; タバコ; RNAポリメラーゼ; 光; 転写因子; 転写制御; プロモータ

Plastids are known to have originated from prokaryotic photosynthetic cyanobacteria. Consequently plastids are semi-autonomous and contain their own genetic system. In higher plants, plastids contain a small prokaryotic-type genome which encodes _120 genes. Higher plant plastids contain two types of RNA polymerases, eubacterial-type PEP (plastid-encoded plastid RNA polymerase) and phage-type NEP (nuclearencoded plastid RNA polymerase). In order to recognize promoters, PEP requires an exchangeable subunit, sigma factor. In bacteria, a variety of sigma factors specifically recognize different promoter sequences. Higher plants also encode multiple sigma factor genes. Chloroplast sigma factors are key regulators of the chloroplast gene expression and chloroplast differentiation. This research revealed the roles of plastid sigma factors in chloroplast differentiation and environmental responses. Firstly, we have revealed that SIG6 is an early general sigma factor in plastids that plays a key role in chloroplast development at an early stage of seedling development. Furthermore, we demonstrated that SIG5 acts as a special sigma factor in chloroplasts that selectively recognizes the psbD LRP in response to various environmental stresses.

已知质体起源于原核光合蓝细菌。因此质体是半自主的并且包含它们自己的遗传系统。在高等植物中，质体含有一个小的原核型基因组，编码_120个基因。高等植物质体含有两种类型的RNA聚合酶，真细菌型PEP（质体编码质体RNA聚合酶）和噬菌体型NEP（核编码质体RNA聚合酶）。为了识别启动子，PEP 需要一个可交换亚基，即西格玛因子。在细菌中，多种西格玛因子特异性识别不同的启动子序列。高等植物还编码多个西格玛因子基因。叶绿体西格玛因子是叶绿体基因表达和叶绿体分化的关键调节因子。这项研究揭示了质体西格玛因子在叶绿体分化和环境响应中的作用。首先，我们发现SIG6是质体中的早期通用西格玛因子，在幼苗发育早期阶段的叶绿体发育中发挥关键作用。此外，我们证明 SIG5 作为叶绿体中的特殊 sigma 因子，选择性识别 psbD LRP 以响应各种环境胁迫。

项目成果

Novel nuclear-encoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana

• DOI: 10.1016/s0014-5793(02)02388-8
• 发表时间: 2002-03-13
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Morikawa, K;Shiina, T;Toyoshima, Y
• 通讯作者: Toyoshima, Y

Morikawa, K., Shiina, T., Murakami, S., Toyoshima, Y.: "Novel nuclear encoded proteins interacting with a plastid sigma factor, Sig1 in Arabidopsis thaliana"FEBS Letters. 314. 300-304 (2002)

Morikawa, K.、Shiina, T.、Murakami, S.、Toyoshima, Y.：“拟南芥中与质体 Sigma 因子 Sig1 相互作用的新型核编码蛋白”FEBS 快报。

Baba, K., Schmidt, J., Espinosa-Ruis, A., Villarejo, A., Shiina, T., Gardestrom, P., Sane, A.P., Bhalerao, R.P.: "The dual targeted organells RNA polymerase RpoT ; 2 of Arabidopsis plays a significant role in root growth and early development of plants"Pl

Baba, K.、Schmidt, J.、Espinosa-Ruis, A.、Villarejo, A.、Shiina, T.、Gardestrom, P.、Sane, A.P.、Bhalerao, R.P.：“双重靶向细胞器 RNA 聚合酶 RpoT；2

葉緑体の形質転換から見える世界:タンパク質の合成から分解

通过叶绿体转化看到的世界：从蛋白质合成到蛋白质降解

• 发表时间: 2003
• 作者: 椎名隆

Organellar gene transcription and early seedling development are affected in the rpoT;2 mutant of Arabidopsis

• DOI: 10.1111/j.1365-313x.2004.02022.x
• 发表时间: 2004-04-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Baba, K;Schmidt, J;Bhalerao, RP
• 通讯作者: Bhalerao, RP