DEVELOPMENT OF MULTIPHASE BIOREACTORS INVOLVING ORGANIC SOLVENT WITH SILICONE-IMMOBILIZED BIOCATALYSTS

涉及有机溶剂和有机硅固定生物催化剂的多相生物反应器的开发

基本信息

批准号：
02650708
负责人：
KAWAKAMI Koei
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The development of supports for immobilized enzymes and microbial cells in non-aqueous systems is an area of high interest. We applied hydrophobic silicone polymer to immobilization by entrapment of biocatalysts operating in organic media.1.Horse liver alcohol dehydrogenase,which was once solubilized in a small amount of water,or impregnated in water-containing porous particles,was immobilized in a silicone polymer sheet. This configuration also enabled to perform a simple packed-bed operation for the enzymatic oxidation of alcohol and reduction of aldehyde with a coupled-substrate coenzyme recycling in organic media.2.Immobilization of Nocardia corallina was examined for epoxidation of liquid alkenes such as 1-tetradecene,1-octene and styrene to produce corresponding epoxides in the presence of n-hexadecane as the organic solvent. The silicone polymer in which droplets of the cell suspension were finely dispersed,was the most suitable material retaining high epoxide productivity. In o … More rder to change hydrophobicity-hydrophilicity balance of the support over a wide range,it was effective to immobilize the cells in a mixed matrix composed of silicone polymer and calcium alginate gel. In the support containing excess silicone over 60%,fine particles of the alginate were dispersed in the continuous silicone phase,and in the case of alginate over 40% the phase configuration was inversed. The optimum composition of the mixed matrix was 80-90% silicone+20-10% alginate for the production of 1,2-epoxytetradecane,40-50% silicone+ 60-50% alginate for 1,2-epoxyoctane,and almost 0% silicone+100% alginate for styrene oxide. This significant change of the optimum composition was closely related to the degree of substrate inhibition. Such an optimized composite-immobilized cells offered the epoxide productivity superior to the free cells in the two-liquid phase after a longer period of batch operation,and enabled to perform a relatively simple operation of a continuous three-phase bioreactor system. Less

非水系统中固定化酶和微生物细胞载体的开发是一个备受关注的领域，我们通过包埋在有机介质中运行的生物催化剂来应用疏水性有机硅聚合物来固定化。1.马肝乙醇脱氢酶，曾经是。溶解在少量水中，或浸渍在含水多孔颗粒中，固定在有机硅聚合物片材中，这种配置也使得能够执行简单的操作。填充床操作，通过偶联底物辅酶在有机介质中回收乙醇和还原醛。 2. 固定化珊瑚诺卡氏菌用于液体烯烃（如 1-十四碳烯、1-辛烯和苯乙烯）的环氧化反应。在正十六烷作为有机溶剂的情况下产生相应的环氧化物，其中细胞悬浮液的液滴在其中。精细分散，是保持高环氧化物生产率的最合适材料。为了在大范围内改变支持物的疏水性-亲水性平衡，将细胞固定在由有机硅聚合物和海藻酸钙组成的混合基质中是有效的。在含有过量硅酮超过60%的载体中，海藻酸盐的细颗粒分散在连续的硅酮相中，并且在海藻酸盐超过40%的情况下，相构型为生产1,2-环氧十四烷的混合基质的最佳组成为80-90%有机硅+20-10%海藻酸盐,生产1,2-环氧辛烷的最佳混合基质组成为40-50%有机硅+60-50%海藻酸盐。 0%有机硅+100%海藻酸盐用于氧化苯乙烯，这一最佳组成的显着变化与底物抑制程度密切相关。这种优化的复合固定化细胞在较长时间的间歇操作后，其环氧化物生产率优于两液相中的游离细胞，并且能够以相对简单的方式进行连续三相生物反应器系统的操作。