Identification and characterization of a novel organelle gathered around centrosomes in a microtubule-dependent manner

以微管依赖性方式聚集在中心体周围的新型细胞器的鉴定和表征

基本信息

批准号：
13680794
负责人：
SHIINA Nobuyuki
金额：
$ 2.3万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680794/
关键词：
mRNA Transport Local translation Synaptic plasticity Staufen FMRP CaM kinase II BDNF p105 中心体 mRNA輸送体樹状突起微小管

项目摘要

mRNA transport and subsequent local translation of the mRNAs in neuronal dendrites are widely believed to be essential for local regulation of synaptic efficacy. The dendritic mRNAs are transported in densely packed granules containing the mRNAs and ribosomes, called RNA granules.RNG105 (RNA granule protein 105) is a novel protein we originally identified as a centrosomes- and microtubule-associated protein. Expression of RNG105 protein in cultured cells induces formation of cytoplasmic granules containing ribosomes and mRNAs. RNG105 was expressed highly in neuronal dendrites. These characteristics of RNG105 protein suggested that the RNG105-induced granules may equivalent to RNA granules in neurons. Immunostaining of rat hippocampal slices showed that RNG105 was associated with granules containing ribosomes and mRNAs in neuronal dendrites. Staufen, a protein for mRNA transport, and FMRP, a protein for translational regulation, were identified as the components of the granule. Microtubule- and actin-dependent motors, kinesin and myosin, were also the components of the granule. Furthermore, mRNAs associated with the granule were identified. Many of the mRNAs, such as CaM kinase IIα, CREB and BDNF mRNAs, were the key molecules for long-term synaptic plasticity. These lines of evidence indicated that RNG105 is a novel protein associated with mRNA-transporting granules which may be responsible for synaptic plasticity in neuronal dendrites.

人们普遍认为，神经元树突中的 mRNA 运输和随后的 mRNA 局部翻译对于突触功效的局部调节至关重要。树突 mRNA 在含有 mRNA 和核糖体的密集颗粒（称为 RNA 颗粒）中运输。RNG105（RNA 颗粒蛋白 105）。）是我们最初鉴定为中心体和微管相关蛋白的一种新蛋白，RNG105 蛋白在培养细胞中诱导表达。含有核糖体和 mRNA 的细胞质颗粒的形成在神经元树突中高表达，RNG105 蛋白的这些特征表明 RNG105 诱导的颗粒可能相当于神经元中的 RNA 颗粒。神经元树突中的核糖体和 mRNA，一种用于 mRNA 运输的蛋白质， FMRP（一种翻译调节蛋白）被鉴定为微管和肌动蛋白依赖性马达的成分，驱动蛋白和肌球蛋白也是颗粒的成分。此外，还鉴定了许多与颗粒相关的mRNA。 CaM 激酶 IIα、CREB 和 BDNF mRNA 等 mRNA 是长期突触可塑性的关键分子。这些证据表明 RNG105 是一种新型突触可塑性分子。与 mRNA 运输颗粒相关的蛋白质，可能负责神经元树突的突触可塑性。