The development of teaching materials to show visually DNA as the 'color-code' genome, entire chromosomes or any of their parts using in situ hybridization technique.

开发教材，使用原位杂交技术以“颜色代码”基因组、整个染色体或其任何部分的形式直观地显示 DNA。

基本信息

批准号：
13680201
负责人：
TAKAHASHI Chigusa
金额：
$ 1.34万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

1.The development of teaching materials to show visually the two parental genomes in the F1 hybrid using genomic in situ hybridization (GISH).GISH was clearly able to distinguish between the two parental genomes in the bigeneric F1 hybrid Gasteria lutzii x Aloe aristata, when genomic DNA of either G.lutzii or A.aristata was used as a probe. The Gasteria genome (four long, three short chromosomes) were fluorescent yellow ; the Aloe genome (four long, three short chromosomes ) fluorescent orange. Using the GISH results, teaching materials to show visually two different genome from different parents in the F1 hybrid were developed.2.The development of teaching materials to show visually meiotic pairing, separation and recombination of chromosomes by genomic in situ hybridization (GISH).Teaching materials to show visually meiotic pairing, separation and recombination of chromosomes in a bigeneric hybrid Gasteria lutzii x Aloe aristata were developed by genomic in situ hybridization (GISH) using the DNA of the original species as a probe. Chromosome painting by GISH enabled the direct and clear study of homoeologous chromosome behavior during the meiotic process. That is, it become possible through color-coding to present visually the following : reduction of chromosome numbers by the bivalent formation and random assortment of paired chromosomes and segregation to both poles at first meiotic division, followed by a separation of chromatids of each chromosome to the opposite poles and transmission of them to the gametes at second meiotic division.Using the pictures provided by GISH, students could understand the mechanism of separation and recombination of chromosomes as different genomes. Then, students could acknowledge that intergenomic recombinations were incorporated into the gametes.3.The development of efficient and reliable protocols, which include a detailed practical guide for student use.

1.教学材料的开发从视觉上显示F1混合动力中的两个父母基因组使用原位杂交（Gish）。Gish显然能够区分幼虫中的f1 Hybrid gasteria lutzii lutzii x aloe Aristata中的两个父母基因组，当时是G..lutzii a.lutzii a a a a.Arista的基因组dna时， Gasteria基因组（四个长，三个短染色体）为荧光黄色。芦荟基因组（四个长，三个短染色体）荧光橙。 Using the GISH results, teaching materials to show visually two different genome from different parents in the F1 hybrid were developed.2.The development of teaching materials to show visually meiotic pairing, separation and recombination of chromosomes by genomic in situ hybridization (GISH).Teaching materials to show visually meiotic pairing, separation and recombination of chromosomes in a bigeneric hybrid Gasteria lutzii x Aloe Aristata是通过基因组原位杂交（GISH）使用原始物种的DNA作为探针开发的。 Gish染色体绘画使在减数分裂过程中对同种染色体行为进行了直接和清晰的研究。也就是说，通过颜色编码以视觉呈现以下内容：通过二价形成和对配对的染色体的随机分类减少和随机分类，并在第一次减数分裂分裂的情况下对两极进行分离，然后将每个染色体的染色体分离到相反的杆子中，并通过隔离的机制来理解，并在隔离的机构中进行了隔离的机制。染色体作为不同基因组的重组。然后，学生可以承认将基因组分组重组纳入了配子中。3。开发高效可靠的协议，其中包括用于学生使用的详细实用指南。