Effects of combined intervention of soy isoflavone and moderate exercise on bone loss in ovariectomized mice

大豆异黄酮联合适度运动对去势小鼠骨丢失的影响

• 批准号: 13680175
• 负责人: ISHIMI Yoshiko
• 金额: $ 2.18万
• 依托单位: National Institute of Health and Nutrition
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680175/
• 关键词: Osteoporosis; Soybean isoflavone; Soymilk; Exercise; Estrogen; 老化促進マウス; 大豆; イソフラボン

Body fat accumulation and bone loss are both often associated with estrogen deficiency following menopause. In this study, we examined whether soy isoflavone, one of the phytoestrogens, and moderate exercise interventions exhibit cooperative effects on body composition and bone mass in ovariectomized (OVX) mice. Eight week-old female mice were assigned to six groups : (1)sham-operated (sham) ; (2)OVX ; (3)OVX with received a soy isoflavone diet (OVX+ISO) ; (4)OVX with exercised on a treadmill (OVX+EX) ; (5)OVX with given both isoflavone and exercise (OVX+ISO&EX) ; and (6)OVX with treated with 17 β-estradiol subcutaneously (OVX+E2). Body composition and bone mineral density (BMD) were estimated by dual-energy X-ray absorptiometry (DXA). After the 6-week intervention, whole body fat (%) in the OVX group showed significantly higher than that in the sham group. Intervention of exercise and isoflavone alone partially inhibited OVX-induced body fat gain, and the combined intervention as well as E2 treatment completely restored fat mass to the sham level. Lean body mass in the whole body was not different in OVX group compared with that in OVX+ISO, OVX+EX and OVX+E2 groups, but it was significantly higher in OVX+ISO&EX than in other groups. BMD of the whole body, lumbar spine or femur showed significantly reduced by OVX, and the bone loss was partially inhibited by intervention of exercise or isoflavone alone. However, the combined intervention completely restored the bone mass to the level of sham, as did E2. Serum total cholesterol was significantly increased by OVX, and which was normalized by the combined intervention or E2 treatment. These results demonstrate that combined intervention of soybean isoflavone and exercise prevented body fat accumulation in the whole body with an increase in lean body mass and restoration of bone mass, and reduced high serum cholesterol in OVX mice.

绝经后，体内脂肪的积累和骨质流失通常都与雌激素缺乏症有关。在这项研究中，我们检查了大豆异黄酮，植物雌激素之一以及中等运动干预措施是否对卵巢切除（OVX）小鼠的身体组成和骨骼质量的中等运动进行了合作。将八个星期大的雌性小鼠分配给六组：（1）假手术（假）； （2）OVX; （3）OVX带有大豆异黄酮饮食（OVX+ISO）； （4）OVX在跑步机上锻炼（OVX+EX）； （5）OVX具有给定的异黄酮和运动（OVX+ISO＆ex）; （6）用17β-雌二醇皮下处理的OVX（OVX+E2）。通过双能X射线绝对能力学（DXA）估算体内组成和骨矿物质密度（BMD）。经过6周的干预后，OVX组中的全身脂肪（％）明显高于假手术组。单独的运动和异黄酮的干预部分抑制了OVX诱导的体内脂肪的增益，以及联合干预以及E2治疗将脂肪量完全恢复到假水平。与OVX+ISO，OVX+EX和OVX+E2组相比，OVX组的瘦体重在OVX组中没有差异，但是OVX+ISO＆EX的瘦体比其他组中的瘦体量明显更高。 OVX的整个身体，腰椎或股骨的BMD显着降低，仅通过运动或单独的异黄酮干预就可以部分抑制骨质。但是，联合干预措施与E2一样，将骨质量完全恢复为假的水平。 OVX显着增加了血清总胆固醇，并通过综合干预或E2治疗进行标准化。这些结果表明，大豆异黄酮的综合干预，运动可以防止体内脂肪的积累，随着瘦体重的增加和骨骼质量的恢复，并降低了OVX小鼠的高血清胆固醇。

项目成果

石見佳子(分担): "食品大百科辞典"食品総合研究所編、朝倉書店. 527-531 (2001)

岩见芳子（撰稿人）：《食品百科全书》，食品研究所编，朝仓书店 527-531（2001）。

Ishimi Y, Yoshida M, Wakimoto S, Wu J, Chiba H, Wang X, Takeda L, Hiyama C: "Genistein, a soybean isoflavone, effects bone marrow lymphopoiesis and prevents bone loss in castrated male mice"Bone. (in press). (2002)

Ishimi Y、Yoshida M、Wakimoto S、Wu J、Chiba H、Wang X、Takeda L、Hiyama C：“染料木黄酮，一种大豆异黄酮，影响骨髓淋巴细胞生成并防止去势雄性小鼠骨质流失”骨。

Wu J, Way XX, Chiba H, Higuchi M, Takasaki M, Ohta A, Ishimi Y: "Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice"J. Appl Physiol.. 94. 335-342 (2003)

Wu J，Way XX，Chiba H，Higuchi M，Takasaki M，Ohta A，Ishimi Y：“运动和金雀异黄素的联合干预可预防雄激素缺乏引起的小鼠骨质流失”J。

Wu J, Wang X, Chiba H, Higuchi M, Yamada K, Ishimi Y: "Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in OVX mice."Metabolism. in press. (2004)

Wu J、Wang X、Chiba H、Higuchi M、Yamada K、Ishimi Y：“大豆异黄酮和适度运动的联合干预可防止 OVX 小鼠体内脂肪升高和骨质流失。”​​代谢。

Ishimi Y, Yoshida M, Wakimoto S, Wu J, Chiba H, Way X, Takodu K, Miyamura C: "Genistein, a Soybean Isoflavone, Affects Bone Mallow Lymphopoiesis and Prevents Bone Loss in Castrated Male Mice"Bone. 31. 180-185 (2002)

Ishimi Y、Yoshida M、Wakimoto S、Wu J、Chiba H、Way X、Takodu K、Miyamura C：“染料木黄酮，一种大豆异黄酮，影响骨髓锦葵淋巴细胞生成并防止去势雄性小鼠骨质流失”骨。