Chemical analysis and immunohistology research on non-collagen protein that takes part in the formation of calcospherites

参与钙球体形成的非胶原蛋白的化学分析和免疫组织学研究

基本信息

批准号：
13671914
负责人：
MISHIMA Hiroyuki
金额：
$ 1.98万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

On the mammal, the mineralization of mantle dentin is formed with the mineralization of the matrix vesicle. But the mineralization of circumpulpal dentin is formed by the mineralization that relates to collagen fibers. The spherical calcospherites were observed in the predentin of crown dentin. In the apical region of root dentin, the calcospherites were not observed and the calcified collagen fibers were observed. The species specificity was detected in the development and distribution of calcospherites. As compared with in the cementum covered lingual dentin of continuously growing teeth such as rat and rabbit incisors, and in the cementum covered root dentin uncontinuously growing teeth such as humans and bovine incisors, the form and the size of calcospherites were greatly different. In the marsupial opossum dentin, the calcospherites were only observed at the cervical region of crown. In the reptile crocodilian dentin, the size of the calcospherites was smaller than that of mammal … More , and the fiber-like structure was observed on the surface of calcospherites. On the Ca/P ratio of calcospherites in human, bovine and opossum, the significant difference had been found between the crown and the root of dentin. The difference was not found on the Ca/P ratio of calcospherites in the continuously growing teeth such as rat and rabbit, and polyphyodont crocodilian. It is considered that the difference of the mechanisms of teeth formation related to that of calcospherites formation. With the results of the analytical electron microscopy, except the main element of calcium and phosphorus, elements of Mg S, K, and Al were detected in the stage of calcospherite formation. It was clarified that Mg especially had the key role the formation of the calcospherites among small amount elements from the observation of the analysis electron microscope A lot of Mg was detected in the first stage of the formation of calcospherites. Mg may contribute to physicochemical stability of the apatite crystal. It seems that Mg takes part in the nuclear formation on the initial stage of calcospherites formation. Moreover, it is considered that the localization of S means the existence of the proteoglycan that is non-collagen protein in the calcospherites. It is judged that S of the sulfuric acid radical in the proteoglycan was detected. The calcospherites of crocodilian were small and S was distributed from the cervical region to the root region of dentin. It was considered that the proteoglycan controlled the formation of calcospherites because it took part in the formation of collagen fibers and has closer affinity with Ca. When a human molar was stained with the antiheparan sulfate antibody, the immune reaction attitude was different between the crown pulp and the root pulp. It is considered that this opinion is related to the difference in the form and development of calcospherites between the crown and the root dentin. The reaction was seen well in the region with which dentin was being firmed in the predentin of crown. The calcospherites existed in the mineralization front of dentin, and it was suggested that the form of calcospherites was related to the localization of the heparan sulfate. Less

在哺乳动物上，通过基质囊泡的矿化形成地幔牙本质的矿化。但是，与胶原蛋白纤维有关的矿化形成了牙本质牙本质的矿化。在牙冠牙本质的前蛋白中观察到球形骨磷酸盐。在根牙本质的顶端区域中，未观察到骨磷酸盐，并且观察到钙化的胶原蛋白纤维。在骨磷酸盐的开发和分布中检测到规格特异性。与牙骨质覆盖了连续生长的牙齿（例如大鼠和兔切牙）的舌齿，以及在牙骨质中覆盖的根牙本质牙本质不断生长的牙齿，例如人类和牛门牙，骨磷岩的形式和大小都大不相同。在有袋牙的牙本质中，仅在冠的宫颈区域观察到骨磷酸盐。在crocodilian牙本质中，骨磷酸盐的大小小于哺乳动物的牙本质……更多，并且在钙磷层的表面上观察到纤维样结构。在人，牛和负鼠中骨磷酸盐的CA/P比上，在牙冠和牙本质根之间发现了显着差异。在持续生长的牙齿（例如大鼠和兔子，多晶状鳄鱼）中，骨磷岩的CA/P比没有发现差异。据认为，牙齿形成机制的差异与骨磷岩形成相关的机制。除了钙和磷的主要元素外，分析电子显微镜的结果是在骨磷岩形成的阶段中检测到Mg S，K和Al的元素。可以澄清的是，MG特别具有关键作用，即从分析电子显微镜观察到少量元素之间的骨磷酸盐形成，在形成Calcospherites的第一阶段中检测到了很多MG。 Mg可能有助于物理圈属矿物很小，S从宫颈区域分布到牙本质的根部区域。据认为，蛋白聚糖控制了骨磷酸盐的形成，因为它参与了胶原纤维的形成，并且与Ca具有更紧密的亲和力。当人摩尔用抗硫酸盐抗体染色时，牙冠和根浆中的免疫反应态度不同。据认为，这种意见与牙冠和根果之间的骨磷岩的形式和发展的差异有关。在Crown的predentin中，牙丁在该区域中均可很好地看到。骨磷岩存在于牙本质的矿化前端，并提出骨磷酸盐的形式与硫酸乙酰肝素的定位有关。较少的