Molecular cloning and characterization of chick sialoprotein associated with cones and rods, a developmentally regulated glycoprotein of interphotoreceptor matrix

与视锥细胞和视杆细胞相关的鸡唾液酸蛋白的分子克隆和表征，这是一种光感受器间基质的发育调节糖蛋白

基本信息

批准号：
13671856
负责人：
ZAKO Masahiro
金额：
$ 2.3万
依托单位：
Aichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

PG-M/versican. The antibody specifically stains the photoreceptor layer, where we recently reported an absence of PG-M/versican. In this study, we re-characterized the antibody and identified the molecule that reacts to MY-174 at the photoreceptor layer. Immnohistochemistry localized the antigen to the matrix surrounding photoreceptors. A variety of glycosidase digestions showed that the antigen is the 150-kDa glycoprotein that has sialylated N- and O-linked glycoconjugates having a molecular mass of more than 30-kDa. The peptide sequences obtained from purified MY-174 antigen showed we had sequenced a full-length cDNA with an open reading frame of 2787 base pairs, encoding a polypeptide of 928 amino acids, with 56 and 54% identities to human and mouse sialoprotein associated with cones and rods (SPACRs), respectively, and with the structural features observed in SPACRs. The specific sialylated O-glycoconjugates here are involved in the epitope structure for MY-174. SPACR first appeared by embryonic days 15-16, and expression increased with developmental age, paralleling the adhesion between neural retina and retinal pigment epithelium. Thus, we concluded that the MY-174 antigen at the photoreceptor layer, a developmentally regulated glycoprotein, is identical to chick SPACR and may be involved in a novel system mediating adhesion between neural retina and retinal pigment epithelium.

PG-M/Versican。该抗体特异性染色染色器层，我们最近报道了不存在PG-M/Versican。在这项研究中，我们重新表征了抗体，并鉴定了在感光层处对MY-174反应的分子。 INMNO组织化学将抗原定位于周围光感受器的基质。多种糖苷酶消化表明，抗原是150 kDa糖蛋白，具有脱脂的N和O连接的糖缀合物，其分子质量超过30 kDa。从纯化的My-174抗原获得的肽序列表明，我们已经对具有2787个碱基对的开放式阅读框对全长cDNA进行了测序，编码了928个氨基酸的多肽，与人和小鼠唾液酸相关的56％和54％的身份锥和杆（SPACR）和圆锥体（SPACR），以及在SPACR中观察到的结构特征。这里的特定溶解的O-糖缀合物参与MY-174的表位结构。 SPACR首先是由胚胎天15-16天出现的，表达随发育年龄的增长而增加，与神经视网膜和视网膜色素上皮之间的粘附平行。因此，我们得出的结论是，感光受体层的My-174抗原是一种发育调节的糖蛋白，与雏鸡的SPACR相同，并且可能参与了一种新型的系统，该系统介导神经视网膜和视网膜色素上皮之间的粘附。