BASIC RESEARCH OF THE GENE THERAPY FOR ENDOMETRIOSIS

子宫内膜异位症基因治疗的基础研究

• 批准号: 13671703
• 负责人: ITO Naoki
• 金额: $ 2.3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671703/
• 关键词: endometriosis; growth factors; receptors; endocrine disruptors; clear cell carcinoma (6); 内分泌撹乱化学物質; 内分泌攪乱化学物質

Endometriosis is reported to be increasing in recent years, and also the relation of endometriosis and dioxin in the animal model of rhesus monkey reported. This project was undertaken to examine the biological aspect of endometriosis for the apply of gene therapy in the near future and make the in vitro model of endometriosis.In the primary culture of endometriosis tissues, various kinds of growth factors and their receptors were checked under the presence of endocrine disruptors (dioxin and bisphenol A). No significant factors were found and in vitro cultures were difficult to be established. Then effects of endocrine disruptors on the proliferation of ovarian cancer cell lines was examined. Two cell lines were used in this project, Caov-3 is adenocarcinoma and ES2 is clear cell carcinoma. Relation of endometriosis and clear cell carcinoma reported in many past papers. Caov-3 proliferated with TCDD, when incubated in the low FBS condition. ES2 proliferation was increased with dioxin inoculation even in normal serum condition. Each ovarian cancer cell line might have its own different responsiveness to dioxin.

据报道，子宫内膜异位症近年来正在增加，并且在恒河猴动物模型中，子宫内膜异位和二恶英的关系也有所增加。该项目是为了检查子宫内膜异位症的生物学方面，以便在不久的将来应用基因治疗，并使子宫内膜异位症的体外模型在子宫内膜异位组织组织的主要培养中，在内分泌干扰物的存在下检查了各种生长因子及其受体（二氧蛋白和双酚A）。找不到重要的因素，很难建立体外培养物。然后检查了内分泌干扰物对卵巢癌细胞系增殖的影响。在该项目中使用了两种细胞系，CAOV-3是腺癌，ES2是透明细胞癌。过去许多论文报告了子宫内膜异位症和清除细胞癌的关系。在低FBS条件下孵育时，CAOV-3与TCDD增殖。即使在正常的血清条件下，ES2增殖也随二恶英接种而增加。每条卵巢癌细胞系可能对二恶英具有不同的反应性。

项目成果

N.Ito, T.Tamaya: "Dioxin and endometriosis"Igaku no Ayumi. 204(13). 959-963 (2004)

N.Ito, T.Tamaya：“二恶英和子宫内膜异位症”Igaku no Ayumi。

S.Hirano, S.Takahashi, N.Ito, T.Tamaya.: "Clinical implication of insulin-like growth factors through the presence of their binding proteins and receptors expressed in gynecological cancers."Eur.J.Gynaec.Oncol.. 25(2). 187-191 (2004)

S.Hirano、S.Takahashi、N.Ito、T.Tamaya.：“胰岛素样生长因子通过其结合蛋白和在妇科癌症中表达的受体的存在而产生的临床意义。”Eur.J.Gynaec.Oncol..

S.Hirano: "Clinical implication of insulin-like growth factors through the presence of their binding proteins and receptors expressed in gynecological cancers"Eur.J.Gynaec.Oncol.. 25・2. 187-191 (2004)

S.Hirano：“妇科癌症中表达的胰岛素样生长因子的结合蛋白和受体的存在的临床意义”Eur.J.Gynaec.Oncol.. 25・2 (2004)。

伊藤直樹: "ダイオキシンと子宮内膜症"医学のあゆみ. 204・2. 959-963 (2003)

伊藤直树：“二恶英和子宫内膜异位症”医学史204・2（2003）。