The effects of general anesthetics on physiologically active peptide in the brain

全身麻醉药对脑内生理活性肽的影响

基本信息

批准号：
13671576
负责人：
SEGAWA Hajime
金额：
$ 0.9万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Clinical Study:The effect of general anesthetics on the pituitary hormones (ACTH and vasopressin) responses in human was studied. It is clarified that isoflurane and sevoflurane did not attenuate the responses but nitrous oxide suppress them. The effect of benzodiadepine on the responses was studied. Midazolam, added to sevoflurane anesthesia, did not influence the responses. These results suggested that these volatile anesthetics have no effect on the stress responses but nitrous oxide has suppressive effect on the stress responses and an augmentation of GABAA action did not influence the stress responsesAnimal Study:To prove the hypothesis that nitrous oxide affects the stress response by an activation of opioid neuron in the brain, the effect of general anesthetics on the Met-enkephalin and Leu-enkephalin concentration in the cerebrospinal fluid of the rabbit was studied, but the anesthetics did not Increase these opiod concentrations. Next, the effects of general anesthetics on the stressresponse were studied by using rat laparotomy model. Laparotomy stimulation increased the plasma ACTH concentraion under isoflurane anesthesia and this response was attenuated by nitrous oxide. By immunohistochemistry, it was clarified that this stimulation also increased the expression of c-Fos in the nucleaus around the third ventricle, especially paraveritricular nucleus, and cerebral cortex from parietal to temporal lobe. However, the difference of the amount of the protein expression among the general anesthetics was still obscure. Futher study is necessary to clarify whether or not nitrous oxide suppresses the stress response through the intrinsic opioids in the brain

临床研究：研究人类对垂体激素（ACTH和加压素）反应在人类中的影响。可以澄清的是，异氟烷和Sevoflurane没有减弱反应，而是氧化二氮可以抑制它们。研究了苯甲二基二苯二甲酸对反应的影响。咪达唑仑加入了七氟素麻醉，并没有影响反应。这些结果表明，这些挥发性麻醉药对压力反应没有影响，但一氧化二硝酸对压力反应具有抑制作用，而加巴作用的增强并没有影响压力反应的研究：证明一二二氮可以通过对压力反应的假说来影响应力反应，从而影响压力反应。大脑中阿片类神经元的激活，全身麻醉药对兔脑脊髓液中的脑脑蛋白酶和leu-enkephalin浓度的影响，但麻醉剂并未增加这些OPIOD浓度。接下来，使用大鼠剖腹手术模型研究了全身麻醉对应激反应的影响。剖腹手术刺激增加了异氟烷麻醉下血浆ACTH浓度，并通过一氧化二氮降低了这种反应。通过免疫组织化学，可以澄清说，这种刺激还增加了第三个心室周围的核中C-FOS的表达，尤其是弗氏核核，以及从顶叶到颞叶的脑皮质。然而，一般麻醉剂中蛋白质表达的量差异仍然晦涩难懂。需要研究以阐明一氧化二氮是否抑制大脑内在阿片类药物的应力反应是必要的。