The pathogenesis of idiopathic scoliosis: Implication for melatonin and its receptor in the spinal deformities of Hereditary Lordoscoliotic Rabbits (LSR)

特发性脊柱侧凸的发病机制：褪黑激素及其受体在遗传性脊柱侧凸兔 (LSR) 脊柱畸形中的意义

• 批准号: 13671548
• 负责人: SEMOTO Yoshihiro
• 金额: $ 2.18万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671548/
• 关键词: Hereditary Lordoscoliotic Rabbit (LSR); melatonin and its receptor; spinal cord; Quantitative RT-PCR; 突発性側弯症; Lordoscoliotic Rabbit(LSR); Lordo scoliotic Rabbit(LSR); RT-PCR

We previously reported radiological and histological studies investigating the etiology of spinal deformities in a breed of Japanese White Rabbit, the Hereditary Lordoscoliotic Rabbit (LSR). These animals develop thoracic lordoscoliosis during growth and as such can be used as a model for human idiopathic scoliosis. While previous studies in chickens have established that pinealectomy produces scoliosis, the cause of the condition is yet to be fully elucidated. Thus, serum melatonin levels in LSRs were measured by radioimmunoassay to investigate the association between melatonin and the spinal deformities in LSR. Serum melatonin levels in LSRs were significantly higher than those of controls for each time point examined for animals aged from 4 through to 16 weeks. This finding suggests that alterations in serum melatonin levels in LSR are the result of a feedback mechanism associated with the defective functioning of melatonin receptors. We detected the expression of melatonin receptor mRNA in rabbit spinal cord. No significant quantitative differences were found in the level of expression of melatonin receptor mRNA in the spinal cord between LSRs and controls. In relation to the present study, we suggest that causes of spinal deformities in LSR could be due to the contribution of melatonin receptors as well as that of altered serum melatonin levels in LSR.

我们以前报道了放射学和组织学研究研究了日本白兔子（遗传性下肠兔兔（LSR））中脊柱畸形的病因。这些动物在生长过程中发展出胸腔骨骨化，因此可以用作人类特发性脊柱侧弯的模型。虽然先前在鸡的研究确定松果体切除术会产生脊柱侧弯，但该病因尚未完全阐明。因此，通过放射免疫测定法测量了LSR中的血清褪黑激素水平，以研究褪黑激素与LSR中的脊柱畸形之间的关联。 LSR中的血清褪黑激素水平明显高于对对照组的对照，每次检查4至16周的动物。这一发现表明，LSR中血清褪黑激素水平的改变是与褪黑激素受体功能有缺陷相关的反馈机制的结果。我们在兔脊髓中检测到褪黑激素受体mRNA的表达。在LSR和对照组之间的脊髓中，在褪黑激素受体mRNA的表达水平中未发现显着的定量差异。关于本研究，我们建议LSR中脊柱畸形的原因可能是由于褪黑激素受体的贡献以及LSR中血清褪黑激素水平改变的原因。

项目成果

Satoshi Sobajima et al.: "Implication for Melatonin and Its Receptor in the Spinal Deformities of Hereditary Lordoscoliotic Rabbits"SPINE. 28 (6). 554-558 (2003)

Satoshi Sobajima 等人：“褪黑素及其受体在遗传性脊柱侧凸兔脊柱畸形中的意义”脊柱。

傍島 聰: "脊柱変形におけるメラトニンレセプターの関与"日本整形外科学会誌. 75,8. s908 (2001)

Satoshi Sobashima：“褪黑激素受体与脊柱畸形的关系”，日本骨科学会杂志 75,8 (2001)。

Satoshi Sobajima et al.: "Implication for Melatonin and Its Receptor in the Spinal Deformities of Hereditary Lordoscoliotic Rabbits"SPINE. 28(6). 554-558 (2003)

Satoshi Sobajima 等人：“褪黑素及其受体在遗传性脊柱侧凸兔脊柱畸形中的意义”脊柱。

Satoshi Sobajima: "Implication for Melatonin in the Spinal Deformities of Hereditary Lordoscoliotic Rabbits"The Journal of the Japanese Orthopaedic Association. 76 (8). s908 (2001)

Satoshi Sobajima：“褪黑激素对遗传性脊柱侧凸兔子脊柱畸形的影响”日本骨科协会杂志。

傍島 聰: "脊柱変形におけるメラトニンレセプターの関与"日本整形外科学会誌. 75, 8. s908 (2001)

Satoshi Sobashima：“褪黑激素受体与脊柱畸形的关系”日本骨科学会杂志 75, 8. s908 (2001)。