Acceleration of angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administration

结合维管束植入和 FGF-2 给药加速坏死骨中的血管生成

• 批准号: 13671511
• 负责人: ISHIDA Osamu
• 金额: $ 0.96万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671511/
• 关键词: FGF-2; osteonecrosis; angiogenesis; treatment; growth factor

The aim of this study is to accelerate the angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administrationTwenty-four Japanese white rabbits were used. A portion of rabbit iliac crest bone was removed as a free bone graft and frozen in liquid nitrogen to ensure complete cellular necrosis. A narrow hole was created in the bone and the graft was placed in the proximal thigh. In group 1, FGF-2 was injected into the hole at a single dose of 100 micrograms, and the saphenous artery and its venae comitantes were passed through the hole of the bone. In group 2, injection of saline into the hole and vascular bundle implantation was used as a control. Neovascularization around the vessel was evaluated at weeks 1 and 2 after vestmentNeovascularization was observed along the implanted vascular bundle. The vessel density gradually increased from week 1 to week 2 in both groups. At 1 week after surgery, the vessel density in group 1 (0.148) was significantly higher than in group 2 (0.050). At 2 weeks after surgery, the mean of the vessel density in group 1 was 0.375 and that in group 2 was 0. 194. This difference was also statistically significant. The length of vessels at 1 week after surgery in group 1 (0.862mm) was significantly higher than in group 2 (0.330mm). At 2 weeks after surgery, the mean of the vessel length in group 1 was 2.625mm and that in group 2 was 1.383mm. This difference was also statistically significantA local single injection of FGF-2 improved surgical angiogenesis in necrotic bone in this study. As FGF-2 is recognized as a potent mitogen for a variety of mesenchymal cells, the combination of vascular bundle implantation and FGF-2 administration may contribute to the treatment of avascular necrosis of bone

这项研究的目的是通过使用血管束植入和使用FGF-2给药来加速坏死骨的血管生成，其中四个日本白兔子。将一部分兔子rest骨作为游离骨移植，并在液氮中冷冻，以确保完全细胞坏死。在骨头中产生了一个狭窄的孔，将移植物放置在大腿近端。在第1组中，将FGF-2注射到单剂量100微克的孔中，而大动脉及其静脉内comitantes通过骨骼的孔。在第2组中，将盐水注射到孔中，血管束植入被用作对照。沿植入的血管束时观察到vestmentneycolulination后，在第1周和第2周评估血管周围的新血管化。两组的血管密度从第1周逐渐增加到第2周。手术后1周，第1组（0.148）的血管密度显着高于第2组（0.050）。手术后2周，第1组的血管密度为0.375，在第2组中为0。194。这种差异也具有统计学意义。第1组（0.862mm）手术后1周的血管长度显着高于第2组（0.330mm）。手术后2周，组1组的血管长度为2.625mm，第2组中的平均值为1.383mm。在这项研究中，这种差异也是统计学上有意义的局部单一注射FGF-2改善了坏死骨的手术血管生成。由于FGF-2被认为是多种间充质细胞的有效有丝分裂原，因此血管束植入和FGF-2给药的组合可能有助于治疗骨骼的血管坏死

项目成果

Atsuo Nakamae: "Acceleration of surgical angiogenesis in necrotic bone with a single injection of fibroblast growth factor-2 (FGF-2)"Journal of orthopaedic research. (in press).

Atsuo Nakamae：“单次注射成纤维细胞生长因子 2 (FGF-2) 加速坏死骨中的手术血管生成”骨科研究杂志。