Biophysic study of maturation and strength of bone induced by callotasis

胼胝体诱导骨成熟和强度的生物物理学研究

基本信息

批准号：
13671515
负责人：
TAKATA Shinjiro
金额：
$ 0.64万
依托单位：
THE UNIVERSITY OF TOKUSHIMA
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671515/
关键词：
callotasis bone mineral metabolism Fourier transform infrared spectroscopy solid-state NMR femur bone strength Pqct コラーゲン熱分析法固体NMR

项目摘要

We studied maturation and strength of bone during distraction osteogenesis in callotasis. Under general anesthesia, a longitudinal skin incision was made of the lateral aspect of the femur to expose femoral shaft. Four self -tapping screws were placed at right angles to the longitudinal axis of the femoral shaft. The screws were clamped to the external fixator. After that, transverse osteotomy was done between 3rd and 4th screws using a hand saw. Distraction started 7 days after operation at a rate of 0.25 mm every 12 hours (0.5mm/day) for 21 days. Biophysic analysis, measurement of bone strength and bone mineral density were performed just after 21 days of distraction, 3 weeks, 6 weeks, 9 weeks, 12 weeks, 15 weeks after distraction.The results obtained in this study showed that bone strength, bone mineral density of both cancellous and cortical bones, periosteal circumference increased with time during callotasis. As for biophysic changes, T1 relaxation time mesured by phosphorus-31 solid-state nuclear magnetic resonance (NMR) increased after 21 days of distraction, which means an increase in bone strength. In addition, mineral to matrix ratio measured by Fourier transform infrared spectroscopy (FTIR) increased during distraction, which suggests that mineral to matrix ratio may be one of important indices of strength and maturation of bone in distraction osteogeneis.Based on these facts, T1 relaxation time by phosphrus-31 solid state NMR and mineral to matrix ratio by FTIR become promising predictors of maturation and strength of bone during distraction osteogenesis.

我们研究了在callotasis中分散成骨的过程中骨骼的成熟和强度。在全身麻醉下，由股骨的侧面纵向切开纵向皮肤，以暴露股骨轴。将四个自发螺钉与股骨轴的纵轴成直角。将螺钉夹在外部固定器上。之后，使用手锯在第三杆和第四螺钉之间进行横向截骨术。分心在手术后7天开始以每12小时（0.5mm/天）的0.25毫米速度开始21天。生物物理分析，骨强度和骨矿物质密度的测量在分散注意力21天后，3周，6周，9周，12周，分散注意力后15周进行。这项研究的结果表明，在Callotasis期间，骨骼强度，骨强度，骨矿物质的骨骼矿物质密度均随着时间的流逝而增加。至于生物物理的变化，磷-31固态核磁共振（NMR）在21天分散注意力后，T1松弛时间增加，这意味着骨强度的增加。此外，通过傅立叶变换红外光谱（FTIR）测量的矿物质与基质比增加了分散注意力的增加，这表明矿物与基质比可能是分散分散术中骨骼强度和骨骼成熟的重要指标之一，这些事实是通过这些事实预测的，phosphrus-31 soligation prophrus-31 nmrix nmrix to nmrix to nmrix sportir fortrix nmrix y mirix cartrix y mirix rigi s y mirix rigiac cartrix rigi atirix rigiac cartrix rigiac cartrix cartrix cartrix cartrix cartrix cartrix。分散成骨的过程中的骨骼强度。