Multilateral Analysis of Angiogenic Factors of Intracranial Dural Arteriovenous Fistulas

颅内硬脑膜动静脉瘘血管生成因素的多方分析

基本信息

批准号：
13671425
负责人：
KUWAYAMA Naoya
金额：
$ 1.15万
依托单位：
TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671425/
关键词：
dural arteriovenous fistula angiogenic factor vascular endothelial growth factor basis fibroblast growth factor platelet derived growth factor 頭蓋骨内硬膜動静脈瘻

项目摘要

1. Measurement of angiogenic factorsThe plasma levels of the VEGF, bFGF, PDGF-AB, and PDGF-BB (peripheral blood and sinus blood sample) were determined using ELISA kits in 9 patients with intracranial dural AV fistulas. The plasma VEGF and bFGF values were within normal level in all patients. The plasma PDGF-AB and BB levels were high in 4, and 6 patients, respectively.2. Immunohistological stein of the cadaveric dura materImmunohistological staining of the cadaveric dura mater was not available due to the destruction of the cell structure.3. Immunohistological staining of the angiogenic factors and their receptors in the involved dura materImmunohistological staining of tie-2, FGFR-1〜2, FGFR-4, PDGF-A, PDGF-B, PDGFR-α, PDGFR-β, VEGF, VEGFR-1〜3 were examined in 3 specimens with dural AV fistula. Tie-2, PDGF-α, VEGF, VEGFR-3 (Flt-4) were positive in the arterial wall in the dura mater. All factors examined were positive in the endothelial layer of the dural sinus. None of these factors were positive in the brain tissue adjacent the AV fistula.4. Antibody and Immunohistological staining of the Chlamydia pneumoniaeThe serum levels of IgG or IgA were high in 4 of 7 patients with dural AV fistula. This positive rate did not differ from the normal control group. The Chlamydia p. was not immunohistologically detected in one specimen examined.

1.血管生成因子的测定采用ELISA试剂盒测定9例颅内硬脑膜动静脉瘘患者血浆VEGF、bFGF、PDGF-AB、PDGF-BB(外周血和窦血样本)的血浆VEGF和bFGF值。所有患者的血浆PDGF-AB和BB水平分别较高，其中4例和6例均在正常水平。2．由于细胞结构被破坏，尸体硬脑膜无法进行免疫组织学染色。3．对受累硬脑膜中的血管生成因子及其受体进行免疫组织学染色。tie-2、FGFR-1~2、FGFR-4的免疫组织学染色。 , PDGF-A, PDGF-B, PDGFR-α, 3例硬脑膜动静脉瘘标本中硬脑膜动脉壁Tie-2、PDGF-α、VEGF、VEGFR-3(Flt-4)检测阳性。所有检查的因素在硬脑膜窦内皮层中均呈阳性。在 AV 瘘管附近的脑组织中，这些因素均未呈阳性。4．肺炎衣原体的免疫组织学染色 7 名硬脑膜 AV 瘘患者中有 4 名血清中 IgG 或 IgA 水平较高，这一阳性率与正常对照组没有差异。