Inhibitory effect of Sialyl Lewis-X oligosaccharide on reperfusion injury -Optimal timing and temperature during cardioplegia-

唾液酸路易斯-X寡糖对再灌注损伤的抑制作用-心脏停搏时的最佳时机和温度-

• 批准号: 13671416
• 负责人: HAYASHIDA Nobuhiko
• 金额: $ 2.11万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671416/
• 关键词: Sialyl Lewis-X; ischemia reperfusion injury; myocardial protection; endothelial function; myocardial metabolism; 温度; 心筋保護液; ミエロペロキシターゼ活性; 顆粒球エラスターゼ

Objective: Effects of supplemental Sialyl Lewis-X analogue, a major ligand for all three selectin family members, during warm blood cardioplegia were expressed in the blood perfused isolated rat heart.Methods: The isolated hearts were arrested for 60 min with warm blood cardioplegia given at 20-min intervals. This was followed by 60 min of reperfusion. The hearts were divided into the following two groups according to the supplemental drugs added to the cardioplegic solution. The Control group (n=6) received standard warm blood cardioplegia. The SLX group (n=6) received warm blood cardioplegia supplemented with Sialyl Lewis-X analogue (60μg/ml). Cardiac function, endothelial function, myocardial metabolism and myocardial myeroperoxidase activity were assessed before and after cardioplegic arrest.Results: Left ventricular developed pressure and dp/dt were significantly (p<0.05) greater and -dp/dt was significantly (p<0.05) lower in the SLX group than the control group during reperfusion. Coronary flow at 15 min of reperfusion and NO production, when acetylcholine chloride was added were significantly (p<0.05) greater in the SLX group than the control group. Myeloperoxidase activity was significantly (p<0.05) lower in the SLX than the control group.Conclusions: The results suggest that selectin-mediated endothelial-leukocyte interactions may play an important role in myocardial ischemia and reperfusion injury. Supplementation of Sialyl Lewis-X analogue during warm blood cardioplegia may provide superior myocardial protection by suppressing leukocyte-endothelial interaction during early reperfusion period.

目的：补充siAllyl Lewis-X类似物的影响，这是所有三个选择的家族成员的主要配体，在温暖的血液心胡子期间，在血液灌注的分离的大鼠心脏中表达。方法：用20分钟的间隔以20分钟的时间间隔用温暖的血液心脏手术，将孤立的心脏捕获60分钟。随后是60分钟的再灌注。根据添加到心骨溶液中的补充药物，将心脏分为两组。对照组（n = 6）接受了标准的温暖血心。 SLX组（n = 6）接受了补充有siAllyl Lewis-X模拟（60μg/ml）的温暖血液心脏。心脏功能，内皮功能，心肌代谢和心肌肌氧化酶活性在心脏肾上腺抑制前后进行了评估。退回：左心室发育的压力和DP/DT的可能性明显更高（p <0.05），而在SLX组中比对照组更大，而-dp/dt则更大，而-dp/dt则比对照组较低。当乙酰胆碱氯化物在SLX组中显着（P <0.05）时，再灌注15分钟时冠状动脉流量与对照组高显着（P <0.05）。 SLX组的骨过氧化物酶活性比对照组明显低（P <0.05）。结论：结果表明，选择素介导的内皮 - 白细胞相互作用可能在心肌缺血和再灌注损伤中起重要作用。在温暖的血液心脏方期间补充siAllyl Lewis-X类似物可以通过在再灌注早期灌注期间抑制白细胞 - 内皮相互作用，从而提供高级心肌保护。

项目成果

N.Hayashida, et l.: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida 等人：“非体外循环冠状动脉旁路移植真的能保护肾功能吗？”循环杂志。

H.Teshima, et al.: "Obstruction of St.Jude Medical valves in the aortic position : Histology and immunohistrochemistry of pannus"J Thorac Cardiovase Surg. (in press).

H.Teshima 等人：“主动脉位置 St.Jude 医疗瓣膜阻塞：血管翳的组织学和免疫组织化学”J Thorac Cardiovase Surg。

H. Teshima, et al: "Obstruction of St. Jude Medical valves in the aortic position: History and immunohistrochemistry of pannus"J Thorac Surg. in press.

H. Teshima 等人：“主动脉位置 St. Jude 医疗瓣膜阻塞：血管翳的历史和免疫组织化学”J Thorac Surg。

N.Hayashida: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida：“非体外循环冠状动脉旁路移植真的能保留肾功能吗？”循环杂志。

H. Hayashida: "Coronary Artery Bypass Grafting in an Adult Case with Kawasaki Disease"Ann Thorac Cardiovasc Surg. 8, 1. 47-50 (2002)

H. Hayashida：“川崎病成人病例的冠状动脉搭桥术”Ann Thorac Cardiovasc Surg。