Prediction of distant metastasis by using reverse transcriptase-polymerase chain reaction for epithelial and variant CD44 mRNA in the blod of the patients with colorectal cancer

利用逆转录聚合酶链反应检测结直肠癌患者血液中上皮和变异CD44 mRNA预测远处转移

基本信息

批准号：
13671249
负责人：
YAMAUE Hiroki
金额：
$ 2.24万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671249/
关键词：
colorectal cancer Molecular diagnosis CD44 mRNA RT-PCR portal vein peripheral blood CD44mRNA 抹消血

项目摘要

Background : RT-PCR has been applied to identify very small numbers of tumor cells. Molecular detection is thought to provide useful information for the clinical management of postoperative adjuvant therapy regimens.Hypothesis : Reverse transcriptase-polymerase chain reaction (PT-PCR) recognition of mRNA coding for carcinoembryonic antigen (CEA), epithelial and variants CD44 and matrix metalloproteinase 7 (MMP-7) in the portal vein and peripheral blood obtained from patients with colorectal carcinoma could predict live or distant metastasis.Design : Prospective consecutive series.Setting : University hospitalPatients and Methods : From 22 patients with colorectal cancer, portal venous blood and peripheral blood were obtained during surgical manipulation. Using cDNA primers specific for CEA, CD44 and MMP-7, RT-PCR was performed to detect tumor cells.Main Outcome Measures : Clinical significance of RT-PCR for epithelial and variants CD44 mRNA in peripheral blood.Results : On the basis of 3 years of follow-up data, two cases whose peripheral blood had CEA and CD44 variants mRNA positive had distant metastasis (lung and spleen). Expression of epithelial and variant CD44 mRNA in peripheral blood was more correlated with the clinical stage than expression of CEA and MMP-7.Conclusion : Molecular detection of epithelial and variants CD44 mRNA in the peripheral blood may be of distant metastatic value for patients with colorectal carcinoma. Molecular detection in the peripheral blood at surgery suggested that systemic hematogenic tumor sell dissemination is early event of distant metastasis.

背景：已应用RT-PCR来识别少量的肿瘤细胞。人们认为分子检测为术后辅助治疗方案的临床管理提供了有用的信息。型：逆转录酶 - 聚合酶链反应（PT-PCR）识别编码mRNA编码的cincinoembryonic抗原（CEA），上皮和变体CD44和Matrix Metallopoteinase 7 （MMP-7）在门静脉和从结直肠癌患者中获得的外围血液中的血液可预测现场或远处转移。设计：预期连续序列。Setting：setting：University Hositepatients and方法：来自22例结直肠癌，门静脉血液和外围血液和外围性血液和外围性血液和外围的患者在手术操作期间获得血液。使用针对CEA，CD44和MMP-7的cDNA引物，进行了RT-PCR以检测肿瘤细胞。伴侣结果指标：RT-PCR对上皮和变体CD44 mRNA在外周血液中的临床意义。回报：根据3的基础：基于3多年的随访数据，两个病例的外周血为CEA和CD44变体mRNA阳性具有远处转移（肺和脾脏）。与CEA和MMP-7的表达相比，上皮和变体CD44 mRNA在外周血中的表达与临床阶段更相关。结论：周围血液中上皮和变异的分子检测CD44 mRNA的分子检测可能是远距离转移值的近叶患者癌。手术时外周血中的分子检测表明，全身血肿肿瘤销售是远处转移的早期事件。