Physiological role of human specific seminal ubiquitinated CD10-CD13 complex enzyme

人精液泛素化CD10-CD13复合酶的生理作用

• 批准号: 13670431
• 负责人: ABE Sumiko
• 金额: $ 0.58万
• 依托单位: FUKUSHIMA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670431/
• 关键词: CD10-CD13 complex; γ-glutamyl transpeptidase active enzyme; human seman; HL60; HeLa; mRNA; MBL

Human semen-specific γ-glutamyl transpeptidase active protein (SEGAP) is a unique enzyme complex of ubiquitinated CD10 and CD13. In our previous study, we examined the potential of SEGAP to induce phosphorylation of tyrosine in HeLa (human cervical carcinoma) and HL60 (human promyelocytic leukemia) cells, and we observed phosphorylation of 110-kDa, 70-kDa and 60-kDa proteins that are thought to be PI3 kinase, Syk/ZAP-70 and Scr family proteins, respectively. These results suggested that SEGAP affects mRNA expressions of cytokines and other signals in HeLa and HL60 cells.In this study, we investigated the changes in mRNA levels in SEGAP-treated HeLa and HL60 cells. IL-1β, IL-8, IL-12, TNF-a, Lyn, Fas and c-fos mRNAs were all expressed in HeLa cells, and their expression levels were not affected by SEGAP. Mannose-binding lectin (MBL) mRNAs were not expressed in control HeLa cells. On the other hand, SEGAP induced MBL mRNA expression in HeLa cells after 15 to 30 min of incubation. Since MBL initiates the lectin complement pathway that mediates the innate immune system, SEGAP may activate the defense system against invading microorganisms. SEGAP did not affect mRNA levels of IL-1β, IL-8, IL-12, TNF-a, Lyn or c-fos in HL60 cells. CT-1 and iNOS mRNAs were not expressed in control HL60 cells. In a manner similar to that in HeLa cells, SEGAP induced CT-1 and iNOS mRNA expressions in HL60 cells after 15 to 30 min of incubation. NO is one of the messenger molecules involved in the bacteria killing process. These results suggest that SEGAP induces lectin, CT-1 and iNOS mRNA expressions in order to protect sperm from invading microorganisms.

人精液特异性 γ-谷氨酰转肽酶活性蛋白 (SEGAP) 是泛素化 CD10 和 CD13 的独特酶复合物。在我们之前的研究中，我们研究了 SEGAP 在 HeLa（人宫颈癌）和 HL60 中诱导酪氨酸磷酸化的潜力。人早幼粒细胞白血病）细胞，我们观察到 110-kDa、70-kDa 和60-kDa 蛋白分别被认为是 PI3 激酶、Syk/ZAP-70 和 Scr 家族蛋白。这些结果表明 SEGAP 影响 HeLa 和 HL60 细胞中细胞因子和其他信号的 mRNA 表达。 SEGAP 处理的 HeLa 和 HL60 细胞中 mRNA 水平发生变化，IL-1β、IL-8、IL-12、TNF-a、Lyn、Fas 和 c-fos mRNA 均表达。在 HeLa 细胞中，SEGAP 的表达水平不受 SEGAP 的影响，而在对照 HeLa 细胞中，SEGAP 则不表达 MBL mRNA。由于 MBL 启动介导先天免疫系统的凝集素补体途径，SEGAP 可能会激活针对入侵微生物的防御系统，SEGAP 不会影响 mRNA 水平。 HL60 细胞中的 IL-1β、IL-8、IL-12、TNF-a、Lyn 或 c-fos 在对照 HL60 细胞中不表达，SEGAP 中的表达方式类似。孵育 15 至 30 分钟后，诱导 HL60 细胞中 CT-1 和 iNOS mRNA 表达 NO 是参与细菌杀伤过程的信使分子之一。诱导凝集素、CT-1 和 iNOS mRNA 表达，以保护精子免受微生物入侵。

项目成果

Abe S., et al.: "Cytokine expression in blood leukocytes of a rat after application of a tourniuet to a hind llimb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 115 (2002)

Abe S.等人：“对后肢应用止血带后大鼠血液白细胞中的细胞因子表达”第五届国际法律医学摘要进展研讨会。

Sumiko Abe, Ikubumi Mizusawa, Katsuyuki Kanno, Atsuko Yabashi, Takashi Honda, Miwako Suto, Mikio Kuraya, Kouichi Hiraiwa: "Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model"Legal Medicine. in press.

Sumiko Abe、Ikubumi Mizusawa、Katsuyuki Kanno、Atsuko Yabashi、Takashi Honda、Miwako Suto、Mikio Kuraya、Kouichi Hiraiwa：“大鼠止血带模型血液白细胞中细胞因子 mRNA 表达模式的变化”法律医学。

阿部すみ子: "前立腺病変による精液特異 γ-glutamyl transpeptidase活性酵素構成ユビキチンの免疫反応性の変化"福島医学雑誌. 51・3. 189-197 (2001)

Sumiko Abe：“前列腺病变引起的精液特异性γ-谷氨酰转肽酶活性酶成分泛素的免疫反应性变化”福岛医学杂志51・3（2001）。

Abe S., et al.: "NOS mRNA expression in a rat dorsal root ganglion after application of a tourniquet to a hind limb"Journal of Forensic Medicine (16^<th> Meeting of the International Association of Forensic Sciences Abstract book). 45. 96 (2002)

Abe S.等人：“对后肢应用止血带后大鼠背根神经节中NOS mRNA的表达”法医学杂志（国际法医学协会第16次会议摘要书）。

Sumiko Abe, Miwako Suto, Osamu Muraki, Mikio Kuraya, Teizo Fujita, Masahide Nakamura, Hirobumi Gunji, Kouichi Hiraiwa: "Immunohistochemical characterization of seminal γ-glutamyl transpeptidase active protein constituent-ubiquitin in prostate disease"Fuku

Sumiko Abe、Miwako Suto、Osamu Muraki、Mikio Kuraya、Teizo Fujita、Masahide Nakamura、Hirobumi Gunji、Kouichi Hiraiwa：“前列腺疾病中精液γ-谷氨酰转肽酶活性蛋白成分-泛素的免疫组织化学特征”Fuku