The biochemical features and functions of NS gene product of influenza C virus

丙型流感病毒NS基因产物的生化特征及功能

• 批准号: 13670293
• 负责人: HONGO Seiji
• 金额: $ 2.24万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670293/
• 关键词: Influenza C virus; NS gene; NS2; virion; incorporation

Recently we identified the 182 amino acid NS2 protein as a protein of 〜22kDa in influenza C virus-infected cells. In this study, it was demonstrated that the NS2 protein of influenza C virus was present in purified virions as follows.1. Western blot analysis with anti-NS2 serum detected the NS2 protein in egg-grown influenza C/Yamagata/1/88 virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate. NS2 was also present either in purified virions of other strains grown in eggs or in purified C/Yamagata/1/88 virions grown in other host cells(MDCK or HMV-II).2. The NS2 protein was detected in influenza C virions purified by using the binding activity either to the rat red blood cells or to anti-Hemagglutinin-esterase(HE) monoclonal antibody-conjugated protein A sepharose.3. The NS2 protein associated with influenza C virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate was completely resistant to the treatment with 200 μg/ml of TPCK-trypsin for 30 min at 37℃.4. These data indicate that the NS2 protein of influenza C virus is present inside the viral envelope.

最近我们在丙型流感病毒感染的细胞中鉴定出约22kDa的182个氨基酸的NS2蛋白。本研究证明丙型流感病毒的NS2蛋白存在于纯化的病毒粒子中，如下： 1．使用抗 NS2 血清进行分析，检测到通过 5%-40% 钾线性梯度离心纯化的鸡蛋生长的丙型流感/Yamagata/1/88 病毒粒子中的 NS2 蛋白NS2 也存在于在鸡蛋中生长的其他菌株的纯化病毒体中或在其他宿主细胞（MDCK 或 HMV-II）中生长的纯化C/Yamagata/1/88 病毒体中。2 在丙型流感病毒中检测到NS2 蛋白。利用与大鼠红细胞或抗血凝素酯酶(HE)单克隆抗体缀合蛋白A琼脂糖凝胶的结合活性纯化病毒颗粒。3．通过5%-40%酒石酸钾线性梯度离心纯化的丙型流感病毒颗粒对200 μg/ml TPCK-胰蛋白酶在37℃处理30分钟完全耐药。4．丙型流感病毒的蛋白质存在于病毒包膜内。

项目成果

Muyanga J. et al.: "Antigenic and genetic analyses of influenza B viruses isolated in Lusaka, Zambia in 1999"Arch. Virol.. 146. 1667-1679 (2001)

Muyanga J. 等人：“1999 年在赞比亚卢萨卡分离的乙型流感病毒的抗原和遗传分析”Arch.

Tsuchiya E. et al.: "Antigenic structure of the haemagglutinin of human influenza A/H2N2 virus."J. Gen. Virol.. 82. 2475-2484 (2001)

Tsuchiya E.等人：“人甲型流感/H2N2流感病毒血凝素的抗原结构”。

Muyanga, J.: "Antigenic and genetic analyses of influenza B viruses isolated in Lusaka,Zambia in 1999"Archives of Virology. 46・9. 1667-1679 (2001)

Muyanga, J.：“1999 年在赞比亚卢萨卡分离的乙型流感病毒的抗原和遗传分析”病毒学档案 46・9 (2001)。

Tsuchiya E. et al.: "Antigenic structure of the haemagglutinin of human influenza A/H2N2 virus"J. Gen. Virol.. 82. 2475-2484 (2001)

Tsuchiya E.等：“人甲型流感/H2N2流感病毒血凝素的抗原结构”J.

Tsuchiya E. et al.: "Role of overlapping glycosylation sequons in antigenic properties, intracellular transport and biological activities of influenza A/H2N2 virus haemagglutmin"J. Gen. Virol.. 83. 3067-3074 (2002)

Tsuchiya E.等人：“重叠糖基化序列在甲型/H2N2流感病毒血凝素的抗原特性、细胞内转运和生物活性中的作用”J.