Development of new propagation and production systems of some edible wild herbs by increasing their growth activity and proliferation potency

通过提高一些食用野菜的生长活性和增殖能力，开发新的繁殖和生产系统

• 批准号: 13660019
• 负责人: TAKAGI Hideaki
• 金额: $ 2.3万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660019/
• 关键词: Hosta; Polygonatum; Smilacina; dormancy; a year-round culture; forcing culture; micropropagation; マイクロプロパゲーション; 休眠; in vitro増殖; 順化; 短日; 成長力の季節的変動; ギョウジャニンニク; チャイブ; オオバギボウシ; コバギボウシ; 休眠誘導

1.Dormancy and its breaking in Hosta(1)Hosta plants were induced to grow continuously by transfer to both 16-hour daylength and 27℃ regardless of the growth stage. When the growth of main axes weakened for bolting, some lateral buds sprouted and developed into lateral shoots. In such a case, H.sieboldii (H.albomarginata) developed several vigorous lateral shoots per plant, but H.sieboldiana few lateral shoots. After chilling H.sieboldiana developed a few vigorous lateral shoots.(2)Dormancy in Hosta was readily induced by exposure to 8-hour daylength, regardless of the growth stage.(3)The lateral buds in H.sieboldiana had deep paradormancy while the main axes maintained vegetative growth., but those in H.sieboldil had shallow or almost no one.2.Breaking bud dormancy of Polygonatum and Smilacina rhizomes(1)P.macranthum rhizomes had very deep dormancy in November or earlier, and P.odoratum ones deep dormancy in October or earlier. Their dormancy was broken by the treatment for about 90 days at 5 However, the dormancy in July or earlier was hardly broken by such a treatment.(2)The dormancy of S japonica rhizomes was also broken by the 5℃ treatment, but the dormancy in October or earlier failed to be broken by such a treatment.3.In vitro proliferation in HostaA great many adventitious shoots were produced by in vitro culturing young seedlings in Hosta. Those microshoots were maintained in vitro for a long term and efficiently propagated. Acclimatization of the plantlets were achieved in two weeks with a high survival rate.4.Applications of the results obtained(1)Establishing the technical system for a year-round culture in Hosta.(2)Establishing some extreme forcing-culture in Polygonatum and Smilacina.(3)Establishing efficient proliferation by micropropagation in Hosta

1.玉簪休眠及其打破 (1)无论处于什么生长阶段，均通过16小时日照和27℃诱导玉簪植株继续生长。在这种情况下，H.sieboldii (H.albomarginata) 每株植物会发育出几个旺盛的侧枝，但 H.sieboldiana 的侧枝很少。冷后，H.sieboldiana 长出一些旺盛的侧芽。(2)无论处于哪个生长阶段，玉簪都容易在 8 小时的日照下诱导休眠。(3)H.sieboldiana 的侧芽具有深度的副休眠。主轴保持营养生长，但黄精的营养生长较浅或几乎没有。2、打破黄精和黄精的芽休眠。菝葜根茎(1)大花白兰在11月或更早有很深的休眠，玉竹在10月或更早休眠，经过5～90天的处理，休眠被打破，但在7月或更早休眠。 (2)5℃处理也能打破粳米根茎的休眠，但10月或更早的休眠未能被5℃处理打破。 3.玉簪的体外增殖 通过体外培养玉簪幼苗，可以产生大量的不定芽，这些微芽可以在体外长期维持，并在两周内实现了幼苗的高效繁殖。 4.成果应用(1)建立玉簪全年栽培技术体系。(2)建立玉竹、黄精等极限胁迫栽培技术体系。 Smilacina.(3)通过微繁殖在玉簪中建立高效增殖

项目成果

高樹英明, 斎藤康子: "ウルイ(ギボウシ)の成長継続の条件と芽休眠の種類間差異"園芸学会雑誌. 73(別1). 129-129 (2004)

Hideaki Takaki、Yasuko Saito：“Urui (hosta) 芽休眠的条件和差异”，日本园艺学会杂志 73（第 1 部分）（2004 年）。

Takagi, H., Y.Saito: "Conditions of growth maintenance and genotypic differences on bud dormancy in Hosta"J.Japan.Soc.Hort.Sci.. 73(Suppl.1). 129 (2004)

Takagi, H., Y.Saito：“玉簪花芽休眠的生长维持和基因型差异的条件”J.Japan.Soc.Hort.Sci.73(Suppl.1)。

高樹英明: "ギボウシの休眠に及ぼすと日長と種間差異の影響"園芸学会雑誌. 72・別1. 112-112 (2003)

Hideaki Takaki：“光周期和种间差异对玉簪休眠的影响”日本园艺学会杂志 72，第 1 部分。112-112 (2003)。

高樹英明, 星野麻美: "オオバギボウシとコバギボウシの休眠生態の比較"園芸学会雑誌. 71(別1). 271-271 (2002)

Hideaki Takaki，Asami Hoshino：“Oobagiboshi 和 Kobagibou 休眠生态的比较”园艺学会杂志 71（第 1 部分）（2002 年）。

Takagi, H., D.Ito: "Effects of daylength and genotypic differences on dormancy in Hosta"J.Japan.Soc.Hort.Sci.. 72(Suppl.1). 112 (2003)

Takagi, H., D.Ito：“日长和基因型差异对玉簪休眠的影响”J.Japan.Soc.Hort.Sci.72(Suppl.1)。