Analytical study on the importance and the mechanism of chloroplast photorelocation movement

叶绿体光重定位运动的重要性及机制解析研究

基本信息

批准号：
13304061
负责人：
WADA Masamitsu
金额：
$ 33.45万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Importance of chloroplast avoidance response in plants.Using different classes of mutant, phot2 and chup1, defective in chloroplast avoidance movement, we show that these mutants are more susceptible to necrotic damage in high light than wild-type plants. We conclude that chloroplast avoidance movement actually reduces the amount of light absorption by chloroplasts, and might thus play an important role in the survival of plants under natural growth conditions.Function of phytochrome 3 in ferns.In some ferns, phototropism and chloroplast photorelocation are controlled by red light as well as blue light, but the photoreceptor had not been identified. We identify a function of phytochrome 3 (phy3), a chimera of phytochrome and phototropin, both for red light-induced phototropism and chloroplast photorelocation. Since phy3 greatly enhances the sensitivity to white light in orienting leaves and chloroplasts, this chimeric photoreceptor may have played a key role in the divergence and proli … More feration of fern species under low-light canopy conditions.CHUP1 function for organellar positioning and movement.CHUP1 encodes a protein containing multiple domains, such as an actin-binding domain and a proline-rich region. The fusion protein of CHUP1 and GFP expressed in leaf cells was targeted to the outer envelope of chloroplasts, suggesting CHUP1 localization in chloroplasts. CHUP1 was found to bind F-actin in vitro. CHUP1 is thus identified as a protein required for organellar positioning and movement in plant cells.Functional domain of PHOT2.The avoidance movement in Adiantum phot2 mutants could be restored by transient expression of non-mutant AcPHOT2 cDNA, indicating that the chloroplast avoidance movement in this fern is mediated by the Acphot2 protein. Further functional analyses of the Acphot2 protein were performed using this transient assay for chloroplast avoidance movement. The results obtained suggest that the LOV2, but not the LOV1, domain of Acphot2 is essential for avoidance movement. Less

植物中叶绿体回避反应的重要性。在叶绿体回避运动中有缺陷的不同类别的突变体，光照2和CHUP1的重要性，我们表明，这些突变体在高光中比野生型植物更容易受到坏死损害的影响。我们包括叶绿体避免运动的生长实际上会降低植物的生长，从而降低了植物的生长，从而使植物的生存量可能降低，从而使植物的生存量可能降低，并且可以使植物的生存量降低，并且可以使植物的生存量降低，并且可以使绿色植物的生存量降低条件。在蕨类植物中的植物色素3的功能。在某些蕨类植物中，光质主义和叶绿体光层化由红光和蓝光控制，但尚未鉴定出光感受器。我们确定了植物色素3（Phy3）的功能，即植物色素和光致蛋白的嵌合体，均用于红色光诱导的光促性和叶绿体光层分配。 Since phy3 greatly enhances the sensitivity to white light in orienting leaves and chloroplast, this chimeric photoreceptor may have played a key role In the divergence and proli … More ferration of fern species under low-light canopy conditions.CHUP1 function for organellar positioning and movement.CHUP1 encodes a protein containing multiple domains, such as an actin-binding domain and a proline-rich region.在叶片细胞中表达的CHUP1和GFP的融合蛋白针对叶绿体的外膜，表明CHUP1在叶绿体中定位。发现CHUP1在体外结合F-肌动蛋白。因此，CHUP1被确定为有机化学定位和植物细胞运动所需的蛋白质。使用此瞬态测定法进行叶绿体回避运动，对ACPHOT2蛋白进行进一步的功能分析。获得的结果表明，Acphot2的LOV2（而不是LOV1）域对于回避运动至关重要。较少的