A role as a zoonotic disease-causing agent of rotavirus

轮状病毒作为人畜共患疾病病原体的作用

基本信息

批准号：
63560302
负责人：
MINAMOTO Nobuyuki
金额：
$ 1.15万
依托单位：
Gifu University, Faculty of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

As a clue for clarifying a participation as a zoonotic disease-causing agent of rotavirus, viral isolation from several animals in close contact with human , and antigenic and genetic analysis of its virus were performed. The isolation of virus was carried out in a number of animal species, including, dogs, cats and pet birds, but contrary to our expectations, so for only three strains were isolated from dogs. Then, antigenic and genetic properties of the PO-13 strain isolated from pigeon were determined to clear relationship between pigeon and human rotaviruses.An attempt to subgroup the PO-13 strain made by indirect immunofluorescent antibody assay used monoclonal antibodies (MAbs) to its VP6. Of 29 MAbs, 5 reacted only with rotaviruses possessed subgroup I antigen. Therefore, It was found that the PO-13 strain has subgroup I antigen. By the cross neutralization test examined between the PO-13 strain with 7 different serotypes of rotavirus, the PO-13 strain was designated serotype 7 … More similarly with turkey rotavirus recovered in Northern Ireland. In the polyacrylamide gel electrophoresis of viral dsRNA, the most striking difference between the pigeon and mammalian rotaviruses is indicated the electrophoretic migration of dsRNA segments 5, 10 and 11. Segment 5 of PO-13 strain migrates more slowly than those of mammalian rotaviruses, and segments 10 and 11 migrate very closely. RNA-RNA hybrid bands obtained between 4 different serotypes of human rotaviruses and ^<32>P-labeled PO-13 probe were observed only in the homologous reaction.These results indicated that the PO-13 strain is not entirely related in serotype and genotype to the prototype human strain representing serotypes 1-4. The findings including the present results and previously reports which hemagglutination inhibition antibody to the PO-13 strain was detected in many human, would suggests the fact that unknown rotavirus resembled antigenically with the PO-13 strain was widespread human and animal populations. Further investigations on the rotavirus isolation from several companion animals are now in progress. Less

作为澄清轮状病毒作为人畜共患疾病病原体参与的线索，从与人类密切接触的几种动物中分离出病毒，并对其病毒进行了抗原和遗传分析。动物物种，包括狗、猫和宠物鸟，但与我们的预期相反，因此仅从狗中分离出三个菌株。然后，确定了从鸽子中分离的 PO-13 菌株的抗原和遗传特性，以明确鸽子之间的关系。和人类尝试通过间接免疫荧光抗体测定对 PO-13 菌株进行亚组，使用针对其 VP6 的单克隆抗体 (MAb)，在 29 种 MAb 中，有 5 种仅与具有 I 亚组抗原的轮状病毒发生反应。 13株具有I亚型抗原。通过PO-13株与7种不同血清型轮状病毒之间的交叉中和试验，将PO-13株命名为PO-13株。血清型 7 …与北爱尔兰发现的火鸡轮状病毒更相似在病毒 dsRNA 的聚丙烯酰胺凝胶电泳中，鸽子和哺乳动物轮状病毒之间最显着的差异是 dsRNA 片段 5、10 和 11 的电泳迁移。PO 的片段 5。 -13 株的迁移速度比哺乳动物轮状病毒慢，并且片段 10 和 11 的迁移非常接近。仅在同源反应中观察到4种不同血清型的人轮状病毒与^ 32 P标记的PO-13探针之间获得的条带。这些结果表明PO-13毒株在血清型和基因型上与原型人并不完全相关。代表血清型 1-4 的菌株。包括目前的结果和之前在许多人体内检测到 PO-13 菌株的血凝抑制抗体的研究结果表明，未知的轮状病毒存在这一事实。与 PO-13 菌株具有相似抗原性的是广泛存在的人类和动物群体，目前正在对从几种伴侣动物中分离出的轮状病毒进行进一步研究。