Neural mechanisma for generation and inhibition of paradoxical sleep

矛盾睡眠产生和抑制的神经机制

• 批准号: 12680797
• 负责人: KOYAMA Yoshimasa
• 金额: $ 2.37万
• 依托单位: Fukushima Medical University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680797/
• 关键词: Paradoxical sleep; Laterodorsal tegmental nucleus; Acetylcholine; Noradrenaline; Serotonin; Histamine; GABA; Glutamate; アセチルコリンニューロン; 脳幹; レム睡眠; ニューロン活動; 神経生理学

To elucidate the neural mechanisms for regulation of paradoxical sleep (PS), activity of the brainstem neurons was recorded from unanesthetized, head- restrained rats during sleep and waking and effect of several transmitters on these neurons were examined.1) Two type of PS-on neurons, cholinergic and non-cholinergic, are involved in generation of PS. Disinhibition from the monoaminergic and GABAergic neurons plays a crucial role in generation of PS. During waking, cholinergic PS-on neurons are largely influenced by GABAergic neurons than by monoaminergic (noradrenergic) ones. Non-cholinergic PS-on neurons receive inhibition from noradrenergic neurons as well as from GABAergic ones.2) During PS, non-cholinergic PS-on neurons are activated by cholinergic neurons, while cholinergic PS-on neurons are driven by glutamatergic input through NMDA and kainite receptors.3) Activity of WP neurons during waking is maintained by histamine through H_l receptor. Noradrenaline and serotonin had inhibitory effect on WP neurons.4) The cholinergic and monoaminergic neurons in the brainstem are activated by orexin, however switching from slow wave sleep to waking or from slow wave sleep to PS are regulated by the brainstem neurons rather than by the orexinergic neurons.

为了阐明调节反常睡眠 (PS) 的神经机制，记录了未麻醉、头部受限的大鼠在睡眠和清醒期间的脑干神经元活动，并检查了几种递质对这些神经元的影响。 1) 两种类型的 PS-神经元，胆碱能和非胆碱能，参与 PS 的生成。单胺能和 GABA 能神经元的去抑制在 PS 的生成中起着至关重要的作用。清醒时，胆碱能 PS-on 神经元在很大程度上受 GABA 能神经元的影响，而不是单胺能（去甲肾上腺素能）神经元的影响。非胆碱能 PS-on 神经元受到去甲肾上腺素能神经元和 GABA 能神经元的抑制。2) 在 PS 过程中，非胆碱能 PS-on 神经元被胆碱能神经元激活，而胆碱能 PS-on 神经元则由通过 NMDA 的谷氨酸能输入驱动3)清醒时WP神经元的活性由组胺通过H_1受体维持。去甲肾上腺素和血清素对WP神经元有抑制作用。4）脑干中的胆碱能和单胺能神经元被食欲素激活，但从慢波睡眠到清醒或从慢波睡眠到PS的转换是由脑干神经元调节的，而不是由食欲素调节的。食欲素能神经元。

项目成果

Koyama Y.: "Firing properties of neurons in the laterodorsal hypothalamic area during sleep and wakefulness"Psychiat.Clin.Neurosci.. 56. 339-340 (2002)

小山 Y.：“睡眠和清醒期间下丘脑外侧区神经元的放电特性”Psychiat.Clin.Neurosci.. 56. 339-340 (2002)

Koyama Y.: "Histamine is a transmitter to maintain tonic firing of mesopontine tegmental cholinergic neurons during wakefulness"Histamine Research in New Millennium. 199-202 (2000)

Koyama Y.：“组胺是在清醒期间维持中脑桥被盖胆碱能神经元的强直放电的递质”新千年组胺研究。

Ninomiya Y.: "Postnatal development of choline acetyltranferase activity in the rat laterodorsal tegmental nucleus"Neurosci.Lett.. 308. 138-140 (2001)

Ninomiya Y.：“大鼠背侧被盖核中胆碱乙酰转移酶活性的产后发育”Neurosci.Lett.. 308. 138-140 (2001)

Yamao Y., Koyama Y., Kawauchi A., Jodo E., Kayama Y. and Miki T.: "Are micturition systems influenced by sleeparousal system?"Psychiat. Clinic. Neurosci.. 52. 259-231 (2000)

Yamao Y.、Koyama Y.、Kawauchi A.、Jodo E.、Kayama Y. 和 Miki T.：“排尿系统是否受到睡眠系统的影响？”精神病学。

Yamao Y.: "Are micturition systems influenced by sleep-arousal system?"Psychiat.Clinic.Neurosci.. 52. 259-231 (2000)

Yamao Y.：“排尿系统是否受到睡眠唤醒系统的影响？”Psychiat.Clinic.Neurosci.. 52. 259-231 (2000)