Neural mechanisma for generation and inhibition of paradoxical sleep

矛盾睡眠产生和抑制的神经机制

• 批准号: 12680797
• 负责人: KOYAMA Yoshimasa
• 金额: $ 2.37万
• 依托单位: Fukushima Medical University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680797/
• 关键词: Paradoxical sleep; Laterodorsal tegmental nucleus; Acetylcholine; Noradrenaline; Serotonin; Histamine; GABA; Glutamate; アセチルコリンニューロン; 脳幹; レム睡眠; ニューロン活動; 神経生理学; Paradoxical sleep; Laterodorsal tegmental nucleus; Acetylcholine; Noradrenaline; Serotonin; Histamine; GABA; Glutamate; アセチルコリンニューロン; 脳幹; レム睡眠; ニューロン活動; 神経生理学

To elucidate the neural mechanisms for regulation of paradoxical sleep (PS), activity of the brainstem neurons was recorded from unanesthetized, head- restrained rats during sleep and waking and effect of several transmitters on these neurons were examined.1) Two type of PS-on neurons, cholinergic and non-cholinergic, are involved in generation of PS. Disinhibition from the monoaminergic and GABAergic neurons plays a crucial role in generation of PS. During waking, cholinergic PS-on neurons are largely influenced by GABAergic neurons than by monoaminergic (noradrenergic) ones. Non-cholinergic PS-on neurons receive inhibition from noradrenergic neurons as well as from GABAergic ones.2) During PS, non-cholinergic PS-on neurons are activated by cholinergic neurons, while cholinergic PS-on neurons are driven by glutamatergic input through NMDA and kainite receptors.3) Activity of WP neurons during waking is maintained by histamine through H_l receptor. Noradrenaline and serotonin had inhibitory effect on WP neurons.4) The cholinergic and monoaminergic neurons in the brainstem are activated by orexin, however switching from slow wave sleep to waking or from slow wave sleep to PS are regulated by the brainstem neurons rather than by the orexinergic neurons.

为了阐明调节矛盾睡眠（PS）的神经机制，检查了脑干神经元的活性，在睡眠和醒来期间，醒醒时，检查了这些神经元对这些神经元的影响。单胺能和GABA能神经元的抑制作用在PS的产生中起着至关重要的作用。在醒来期间，胆碱能PS-ON神经元在很大程度上受GABA能神经元的影响，而不是受到单胺能（Nor腺能）（甲胺能）。 Non-cholinergic PS-on neurons receive inhibition from noradrenergic neurons as well as from GABAergic ones.2) During PS, non-cholinergic PS-on neurons are activated by cholinergic neurons, while cholinergic PS-on neurons are driven by glutamatergic input through NMDA and kainite receptors.3) Activity of WP neurons during waking is通过组胺通过H_L受体维持。去甲肾上腺素和5-羟色胺对WP神经元具有抑制作用。4）脑干中的胆碱能和单胺能神经元被Orexin激活，但是从慢波睡眠转换为醒来或从慢波睡眠转变为PS，由脑干神经元调节，而不是由脑肌动蛋白能神经元，而不是由Orexin能神经元调节。