Systematic Studies on Host and Endosymbiont of a Green Dinoflagellate

绿色甲藻宿主与内共生体的系统研究

• 批准号: 63540554
• 负责人: WATANABE Makoto M.
• 金额: $ 1.47万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540554/
• 关键词: Green dinoflagellate; Lepidodinium viride; Taxonomy; Ultrastructure; Body scale; Endosymbiont; Carotenoid; Prasinophyceae; 鱗片; 共生体; ペリクル層; 色素; 共生緑色渦鞭毛藻; Lepidodinium viridis; HPLC

A further description of the green dinoflagellate, strain Y-100, with a chlorophyll a- and b- containing endosymbiont is given with special emphasis on the morphology of the host cell. The host dinoflagellate cell is unarmored and has a gymnodinioid overall appearance. The theca or amphiesma basically consists of the outer membrane and flattened thecal vesicles in which no thecal plates are developed. Unusual hand basket-shaped scales cover the entire cell surface together with a layer of mucillaginous material. These findings suggest that the organism is a new member of the Gymnodiniaceae (Gymnodiniales) ; We propoes the name Lepidodinium viride gen. et sp. nov. The ultrastructure of the host cell is basically typical of the dinoflagellates. However, the organism has 1) an usual cytoplasmic projection that may be a homologue of the peduncle, 2) a single membrane-bounded body containing membranous sheets, closely situated next to the endosymbiont, and 3) an electron opaque network-forming appendage surrounding the transverse flagellum. None of these features have been found in other dinoflagellates.Major carotenoids of a endosymbiont in a green dionflagellate, Lepidodinium viride have been analyzed using high-performance liquid chromatography (HPLC). The endosymbiont contains the usual chlorophytes carotenoids, that are neoxanthin, violaxanthin, antheraxanthin and beta-carotene. In addition to thses carotenoids, the two unknown peaks were detected. One peak of the tow is situated at the identical retention time to that of lutein, however, the absorption spectra is diffrent from that of lutein obtained from spinach leaves. Another peak cannot be identified becaues it has quite low absorbance, but seems to be identical to that of zeaxanthin. Based on these results, it is speculated that a prasinophyte would seem to be likeliest candidate for a proqenitor of the endosymbiont.

对含有叶绿素 a 和 b 的内共生体的绿色甲藻菌株 Y-100 进行了进一步描述，特别强调了宿主细胞的形态。宿主甲藻细胞无装甲，整体外观呈裸甲藻样。膜或无膜基本上由外膜和扁平的膜囊泡组成，其中不发育膜板。不寻常的手篮状鳞片覆盖整个细胞表面以及一层粘质物质。这些发现表明该生物体是裸甲藻科（Gymnodiniales）的新成员；我们建议将其命名为 Lepidodinium v​​iride gen。等十一月宿主细胞的超微结构基本上是甲藻的典型特征。然而，该生物体具有1）通常的细胞质投射，可能是花序梗的同源物，2）包含膜片的单个膜边界体，紧邻内共生体，3）周围有电子不透明的网络形成附属物横鞭毛。这些特征在其他甲藻中均未发现。绿色甲藻、绿色鳞甲藻中内共生体的主要类胡萝卜素已使用高效液相色谱 (HPLC) 进行了分析。内共生体含有常见的叶绿素类胡萝卜素，即新黄质、紫黄质、蒽黄质和β-胡萝卜素。除了这些类胡萝卜素之外，还检测到两个未知峰。丝束的一个峰位于与叶黄素相同的保留时间处，然而，吸收光谱与从菠菜叶中获得的叶黄素的吸收光谱不同。无法识别另一个峰，因为它的吸光度相当低，但似乎与玉米黄质的相同。基于这些结果，推测绿藻植物似乎是内共生体祖先的最有可能的候选者。

项目成果

Watanabe et al.,: "Major carotenoid of a chlorophyll aーand bーcontaining endosymbiont in a green dinoflagellnte,Lepidodinium viride (Gymnodiniales,Dinophyta)." Jpn J.plycol.

Watanabe 等人：“绿色甲藻、绿色鳞甲藻（Gymnodiniales，Dinophyta）中含有叶绿素 a 和 b 的内共生体的主要类胡萝卜素。”

Watanabe, M. M., Suda, S., Sasa, T., Inouye, I. & Chihara, M.: "Major carotenodids of a chlorophyll a- and b-containing endosymbiont in a green dinoflagellate, Lepidodinium viride (Gymnodini" Jpn. J. Phycol.

渡边，M.M.，须田，S.，莎莎，T.，井上，I.

Suda,S.,Watanabe,M,M.& Inouye,I: "Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae)" J.Phycol. 25. 596-600 (1989)

须田，S.，渡边，M，M。

Suda, S., Watanabe, M. M. & Inouye, I.: "Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae)." J. Phycol. 25. 596-600 (1989)

须田，S.，渡边，M.M.

Watanabe, M. M., Suda, S., Inouye, I., Sawaguchi, T. & Chihara, M.: "Lepidodinium viride gen. et sp. nov (Gymnodiniales, Dinophyta), a green dinoflagellate with a chlorophyll a- and b-containing" J. Phycol. 26. 741-751 (1990)

渡边，M.M.，须田，S.，井上，I.，泽口，T.