Search for the New Biological Response Modifiers Targeting on TNFα from the Marine Invertebrates

寻找针对海洋无脊椎动物 TNFα 的新生物反应调节剂

基本信息

批准号：
12672055
负责人：
MIYAMOTO Tomofumi
金额：
$ 2.05万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Two norcembrane diterpenes, norcembrenolide and sinuleptolide were identified from the Okinawan soft coral (Sinularia sp.), and they demonstrated a potent inhibitory effect on LPS-induced TNFα production by RAW 264 cells.^<1)> A cembrane diterpene, flexibilide (= sinularine) was identified from the Okinawan soft coral (Sinularia flexibilis), and it demonstrated a potent inhibitory effect on TNFα by L929 cells. Five new glycosphingolipids were isolated from the marine sponge (Amphimedon sp.), and they demonstrated a promoting effect on TNFα production by RAW 264 cells.TNFα is well known substance to modulate the angiogenesis. A new anthraquine, bryoanthrathiophene was isolated from the bryozoan (Watersipora subtorquata d' Orbigny, 1852), and it demonstrated the most potent antiangiogenic activity on BAE cells.^<3)> An antiangiogenic purine analog, 1,3-dimethylisoguaninium was identified from the Okinawan marine sponge (Amphimedon paraviridis).^<6)>TNFα is one of the potent death-factors. A new abeo-sterol, orostanal was isolated form the marine sponge (Stelletta hiwasaensis), and orostanal and its synthetic analog induce apoptosis in human acute promyelotic leukemia cells.^<4)>During the term of this project, a new multidrug resistance modulator, irciniasulfonic acid was isolated from the marine sponge (Ircinia sp.).^<5)> A new cytotoxic diterpene, (-)-axinyssene was isolated from the marine sponge (Axinyssa sp.).^<2)>

从冲绳软珊瑚 (Sinularia sp.) 中鉴定出两种正西膜二萜：去甲西膜内酯和西努肽内酯，它们对 RAW 264 细胞 LPS 诱导的 TNFα 产生具有有效的抑制作用。^<1)> 一种西膜二萜，flexibilide (= sinularine）是从冲绳软珊瑚（Sinularia flexibilis）中鉴定出来的，它表现出强大的从海洋海绵（Amphimedon sp.）中分离出五种新的鞘糖脂，它们对 RAW 264 细胞产生 TNFα 具有促进作用。TNFα 是众所周知的调节血管生成的物质。 , bryoanthrathiophene 是从苔藓虫 (Watersipora subtorquata d' Orbigny, 1852)，并且它表现出对 BAE 细胞最有效的抗血管生成活性。^<3)> 从冲绳海绵 (Amphimedon paraviridis) 中鉴定出一种抗血管生成嘌呤类似物 1,3-二甲基异鸟鎓。^<6)>TNFα 是一种从海绵（Stelletta hiwasaensis）中分离出一种新的阿贝甾醇，奥罗斯塔醛，以及orostanal 及其合成类似物诱导人急性早幼粒细胞白血病细胞凋亡。^<4)>在本项目期间，从海绵 (Ircinia sp.) 中分离出一种新的多药耐药性调节剂 irciniasulfonic Acid。^<5) > 从海绵 (Axinyssa sp.) 中分离出一种新的细胞毒性二萜 (-)-axinyssene。^<2)>

项目成果

期刊论文数量（16）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

T.Miyamoto et al.: "Bryoanthrathiophene, a New Antiangiogenic Constituent from the Bryozoan Watersipora subtorquata (d'Orbigny, 1852)"Journal of Natural Products. 65(9). 1344-1345 (2002)

T.Miyamoto 等人：“Bryoanthrathiophene，一种来自苔藓动物 Watersipora subtorquata 的新抗血管生成成分（dOrbigny，1852）”天然产物杂志。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Miyamoto, T., Kodama, K., Aramaki, Y., Higuchi, R., Soest, R. W. M. V.: "Orostanal, a Novel Abeo-sterol inducing apoptosis in leukemia cell from a marine sponge Stelletta hiwasaensis"Tetrahedron Lett.. 42. 6349-6351 (2001)

Miyamoto, T.、Kodama, K.、Aramaki, Y.、Higuchi, R.、Soest, R. W. M. V.：“Orostanal，一种新型 Abeo-sterol，可诱导海洋海绵 Stelletta hiwasaensis 的白血病细胞凋亡”Tetrahedron Lett.. 42。