Study of neuropathic pain for rats

大鼠神经病理性疼痛的研究

• 批准号: 12671978
• 负责人: UEDA Yutaka
• 金额: $ 1.98万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671978/
• 关键词: Neuropathic pain; Neural symptom; Behavior test; 神経因性疼痛; サイトカイン

The mechanisms of neuropathic pain are complicated. We investigated differences in three animal models on Sprague-Dawley rats using a behavior test and treatment of cilostazol. The diabetic group received an injection of streptozotocin, and the control rats received an injection of 0.1 M citrate buffer. Operations were done on Chronic Constriction Injury (CCI) rats in the same way as with Bennetts' model. The control rats, which received bilateral sham procedures, were used as non-CCI-CFA rats. Operations were done on Complete Freund's Adjuvant (CFA) rats as prescribed by Eliav's model. Each group of diabetes mellitus (DM) rats, non-DM rats, CCI rats, non-CCI-CFA rats, and CFA rats were subdivided into two groups. One group was given pellets containing cilostazol and the other group was given only pellets. All rats were given behavior tests each week. The tests investigated mechano-allodynia, cold allodynia and mechano-hyperalgesia. Mechano-allodynia was induced in all models. Cilostazol was effective on mechano-allodynia only in the DM group. Cold allodynia and mechano-hyperalgesia was induced only in the CCI group, which showed no change with cilostazol. We defined the mechanisms, duration of effect, and nerves for each models. It is important that we deal with each case individually and that understand mechanisms such as neuropathic pain cannot be treated simply

神经性疼痛的机制很复杂。我们通过行为测试和cilostazol的治疗来研究Sprague-Dawley大鼠三种动物模型的差异。糖尿病组接受了链霉菌素的注射，对照大鼠接受了0.1 M柠檬酸盐缓冲液。手术是在慢性收缩损伤（CCI）大鼠上进行的，其方式与Bennetts的模型相同。接受双侧假手术的对照大鼠用作非CCI-CFA大鼠。按照Eliav的模型规定的完整弗朗德辅助（CFA）大鼠进行操作。每组糖尿病（DM）大鼠，非DM大鼠，CCI大鼠，非CCI-CFA大鼠和CFA大鼠分为两组。给一组含有西洛唑唑的小组，另一组仅给出颗粒。每周对所有大鼠进行行为测试。这些测试研究了机械差异，冷呼吸症和机械性毛毛虫。在所有模型中均诱导了机械差异。 Cilostazol仅在DM组中有效地对机械差异有效。 CCI组仅诱导冷异质性异常和高疗法，这表明西洛唑唑没有变化。我们定义了每个模型的机制，效果持续时间和神经。重要的是，我们要单独处理每种情况，并且了解诸如神经性疼痛之类的机制不能简单地治疗

项目成果

Naomi MORI, Yasushi SAKUMA and Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. 30(in printing). (2002)

Naomi MORI、Yasushi SAKUMA 和 Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报。

Naomi MORI, Yasushi SAKUMA, Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. (発表予定).

Naomi MORI、Yasushi SAKUMA、Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报（待出版）。

Naomi MORI, Yasushi SAKUMA, Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. 36(1). (2002)

Naomi MORI、Yasushi SAKUMA、Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报。