Study of skin reconstruction by using the technique of tissue engineering

利用组织工程技术重建皮肤的研究

• 批准号: 12671754
• 负责人: KUMAGAI Norio
• 金额: $ 2.43万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671754/
• 关键词: fibrin; keratinocyte; culture; scaffold; tissue engineered epithelium; tissue engineered dermis; tissue engineered skin; 真皮; 再生組織; 培養表皮; 線維芽細胞; ゼラチン

In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.

在这项研究中，使用纤维蛋白和其他纤维蛋白设计了组织工程的皮肤。将角质形成细胞培养到纤维蛋白凝胶基质上，但角质形成细胞分泌的蛋白酶很容易消化纤维蛋白凝胶。因此，纤维蛋白凝胶基质作为支架的机械强度不稳定。当将一种可溶性结缔组织材料（申请专利）混合在纤维蛋白凝胶基质中时，保护角质形成细胞的蛋白酶消化。此外，通过使用新型的纤维蛋白支架，皮肤成纤维细胞可以在凝胶基质中培养，然后在凝胶基质上培养。从这些结果中，这种新型的纤维蛋白支架可能是有用的装置，例如组织工程的皮肤。现在，我们试图通过在临床上使用新型脚手架来应用于该组织工程的皮肤。