The morphological and functional change of the gap junction and the effect of irsogladine maleate in the peripheral nerve regeneration

间隙连接的形态和功能变化及马来酸伊索拉定在周围神经再生中的作用

基本信息

批准号：
12671427
负责人：
KITAMURA Hirokazu
金额：
$ 2.18万
依托单位：
OITA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The research in this fiscal year did next fact that "The morphological analysis of the Cx32 expression in rat normal peripheral nerve." by continueing in last year. It is already reported that Cx32 signal was recognized at the myelin sheath of peripheral nerve near the node of Ranvier by the fluorescence microscope. In this fiscal year, the peripheral nerve was observed by transmission electron microscope comparison to the immunoelectron microscopy image using the HAM8 antibody which recognized Cx32 was carried out.In the observation of the super micro localization of the gap junction in normal peripheral nerve, the artifact of the vortex state was seen in the myelin sheath even if using the various fixative solution, embedding agent, and temperature etc. And, It also continues on the change of the Cx32 expression in orthograde degeneration provocation peripheral nerve at present, the examination is being carried out.In the meantime, support of this study subsidy, the research of the C … More x32 expression in the lachrymal gland organization was also carried out beingly which Cx32 equal to the peripheral nerve has manifested the gland tissue. Immunofluorescent stain and immunoelectron microscopy image using the HAM8 antibody were observed on rat lachrymal gland tissue slice and primary cultured cells of the gland, and the localization of the gap junction was clarified. It was revealed that two types of gap junctions existed at rat lacrymal gland tissue in vivo. One was the intercellular type between adjacencies gland cell and the other was reflexive type of gap junction between self cell membrane. Later type was clarified where it had been formed in basal plane. In the experimental result of in vitro using the HAM8 antibody, the time-dependent transfer of the gap junction spot was clarified. By the and, transmission electron microscope, they were revealed that the reconstruction cell aggregate had the structure which is morphologically differentiated and intercellular1 gap junction existed between adjacent lacrymal gland cells. It was morphologically shown that this was functionally committed even in the reconstruction lachrymal gland cell.The subsidy was put in consumable stores, etc. without purchasing image recording personal computer set applied in the relation with the delivery amount in the purchase schedule as this fiscal year furniture. Less

大鼠正常周围神经中CX32表达的形态分析。通过透射电子显微镜与Imm unoeelectron显微镜图像进行比较，在正常周围神经中进行了间隙的超级微定位，识别CX32的HAM8抗体。等等，而且，它还继续延续了CX32表达在矫正变性挑衅中的变化，目前，目前，脑膜化的手段，对这项研究的支持补充，C ... L腺体的研究也被携带在哪种CX32等于外围神经的情况下，使用HAM8抗体在大鼠lachrymal腺体组织的细胞上观察到了腺体组织，并使用HAM8抗体观察到了免疫荧光染色。在体内粘液腺组织是腺细胞之间的细胞间类型，而另一个是在基础平面中形成的，而在体外形成了后来的缝隙。通过透射电子显微镜阐明了间隙点的时间依赖性转移，即重建细胞均匀的群落具有形态分化的前骨间隙连接，在JECENT的脱粒腺细胞之间被形成了形式显示，即使在cachrymal cell中官能发挥作用。补贴是将PUA放入耗尽的矿石中，而无需购买图像记录的个人套件，而在购买时间表中以tisc al y Furniture的身份进行了容忍