Analysis of Neonatal Hemostatic and Thrombotic Mechanism, and Exploring of the New Therapeutic Approach.

新生儿止血血栓机制分析，探索新的治疗途径。

基本信息

批准号：
12671067
负责人：
TAKAHASHI Yukihiro
金额：
$ 2.37万
依托单位：
Nara Medical University.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671067/
关键词：
Thrombelastogram neonate actibated factor VII von Willebrand factor von Willebrand factor cleaving protease shear induced platelet aggregation ecto-ATPase Anti-thrombotic neonatal central catheter Thrombelastogram neonate shear induced

项目摘要

Neonatal hemoatatic mechanism was studied using thrombelastgram (TEG). In the early neonatal period, the r and k values of TEG in neonatal whole blood shortened and the ma value of TEG enlarged rather than these values in adult's whole blood. This characterized hypercoagulation prophile of TEG in neonate was able to be recreated by the addition of activated coagulation factors, such as thrombin, activated factor VIIa, ADP or epinephrine into normal adult whole blood. One of data, the results of activated factor VIIa, was recently submitted to the journal " Thrombosis and Haemostatic" enclosed in the booklet. And another paper concerning about the relationship between the hyper coagulation and anti-thrombotic drugs was recently subscribed. The bleeding time and coagulation time were almost normal in neonate despite the low function of platelets and the low concentrations of vitamin K dependent coagulation factors in neonate compared with adults. But, in pathological state, the thrombosis is more frequent in neonate than in children after neonate. The knowledge of the haemostatic mechanism is still obscure in neonate. From our recent study, von Willebrand factor (vWF) was relatively high and von Willebrand factor-cleaving protease was low in cord bloods of low birth weight to full term neonate. Furthermore, the higher multimer of vWF was detected. This higher multimer of vWF is more potent in hemostasis. And it may be suggested that it is related to be near normal bleeding time in neonate. We have also studied on the shear dependent platelet aggregation in cord bloods. And the low and high shear stress platelet aggregation were low, this mechanism were analyzed by the recreated blood components and the measurement of ecto-ATP activity. Ecto-ATPase in cord bloods was higher than that in adult blood. It may be suggested that ecto-ATP ase is related to the anti-thrombotic mechanism.

使用血栓形成（TEG）研究了新生儿发出的机理。在新生儿初期，新生儿全血的TEG的R和K值缩短了，而TEG的MA值则扩大，而不是成人全血的这些值。通过加入激活的凝血因子，例如凝血酶，激活因子VIIA，ADP或肾上腺素，可以重现这种特征在新生儿中预言的特征性高凝液。一个数据，即激活因子VIIA的结果，最近被提交给小册子中封闭的“血栓形成和止血”杂志。最近订阅了有关高凝结和抗凝血药物之间关系的另一篇论文。尽管血小板的功能低，但与成年人相比，新生儿的出血时间和凝血时间几乎正常，而在新生儿的浓度较低。但是，在病理状态下，新生儿的血栓形成比新生儿后的儿童更频繁。在新生儿中，对止血机制的知识仍然晦涩难懂。从我们最近的研究中，冯·威勒德因子（VWF）相对较高，von willebrand因子裂开的蛋白酶的蛋白酶在低出生体重至全年新生儿的脐带血中很低。此外，检测到VWF的较高多聚体。这种较高的VWF多聚体在止血中更有效。可以建议它与新生儿的正常出血时间有关。我们还研究了绳索血液中剪切依赖的血小板聚集。低剪切应力血小板聚集较低，通过重新创建的血液成分和ECTO-ATP活性的测量来分析该机制。绳索血液中的ECTO-ATPase高于成年血液中的ATPase。可以建议ecto-atp ASE与抗栓性机制有关。