GENOMIC DISCORDANCE BETWEEN MONOZYGOTIC TWINS DISCORDANT FOR AUTISM

单卵双胞胎之间的基因组不一致导致自闭症

• 批准号: 12670945
• 负责人: TSUJITA Takahiro
• 金额: $ 2.5万
• 依托单位: DIVISION OF NEUROPSYCHIATRY, DEPARTMENT OF TRANSLATIONAL MEDICAL SCIENCES NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670945/
• 关键词: AUTISM; MONOZYGOTIC TWINS; METHYLATION; EPIGENETICS; RLGS (Restriction Landmark Genome Scanning); FRGP (Fluorescent Representational Genomic Profiling); DNAメチル化

Genomic DNA of monozygotic twins discordant for autism was analyzed to determine whether their genomes were truly identical. The subjects were monozygotic female twins, one of whom had DSM-IV Autistic disorder. Genomic DNA was extracted from leukocytes and was applied to FRGP (Fluorescent Representational Genomic Profiling) analysis. After comparisons of patterns with approximately 600 spots, we detected at least four spots with intensities that obviously differed in the two twins. The discrepancies likely were generated by differences in the methylation status of DNA between the twins. The difference may influence the transcription level of one or more genes.

分析了单卵双胞胎对自闭症的基因组DNA，以确定其基因组是否真正相同。受试者是单卵母亲双胞胎，其中一个患有DSM-IV自闭症疾病。从白细胞中提取基因组DNA，并将其应用于FRGP（荧光代表性基因组分析）分析。在比较了大约600个斑点的模式后，我们发现了至少四个斑点，其强度在两个双胞胎中显然有所不同。差异可能是由于双胞胎之间DNA的甲基化状态的差异而产生的。差异可能会影响一个或多个基因的转录水平。

项目成果

藤丸浩輔 他: "一卵性双生児のゲノムの不一致について"分子精神医学. 2. 260-261 (2002)

Kosuke Fujimaru 等人：“同卵双胞胎中的基因组不一致”分子精神病学。2. 260-261 (2002)

岡崎祐士 他: "精神分裂病の発達的脆弱性形成とエピジェネティックス"脳と精神の医学. 12. 293-302 (2001)

Yuji Okazaki 等人：“精神分裂症的发育脆弱性形成和表观遗传学”《脑与精神病学医学》。12. 293-302 (2001)

TAKAHIRO TSUJITA: "EPIGENETICS IN SCHIZOPHERENIA. Key Word Psychiatry Ver.3"SENTAN-IGAKUSYA. (in press)

TAKAHIRO TSUJITA：“精神分裂症的表观遗传学。关键词精神病学 Ver.3”SENTAN-IGAKUSYA。

Matsumoto S. et al.: "HLA classl distribution in Japanses patients with schizophrenia"Am. J. Med. Genet.. 114. 42-45 (2002)

Matsumoto S. 等：“日本精神分裂症患者的 HLA classl 分布”Am。

Fujimaru K., et al.: "Minor Physical Anomalies in Japanese Patisents with Schizophrenia"Acta Med. Nagasaki. 47. 133-137 (2002)

Fujimaru K.等：“日本精神分裂症患者的轻微身体异常”Acta Med。