Dendritic cell . Immunotherapy following autologous peripheral blood stem cell transplantation for patients with childhood cancer

树突状细胞。

• 批准号: 12670755
• 负责人: WATANABE Tsutomu
• 金额: $ 2.11万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670755/
• 关键词: childhood cancer; Autologous peripheral blood stem cell transplantation; Dendritic cell; Immunotherapy; 造血幹細胞移植; 末梢血幹細胞移植; 小児がん

We investigated the feasibility of dendritic cell immunotherapy following autologous peripheral blood stem cell transplantation for patients with childhood cancer. In 2000, we were able to obtain functional dendritic cells from the CD34^+ cell fraction of the thawed peripheral blood stem cell products by culturing with interleukin (IL)-3, granulocyte/macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-α, and from the CD14^+ cell fraction of them by culturing with IL-4 and GM-CSF. In 2001, we were able to obtain functional dendritic cells from the thawed cord blood cell units, although the number of obtained cells was small. Furthermore, we were able to isolate dendritic cells from the apheresed products, which were mobilized with G-CSF, by using the Mini MACS isolation kit. In 2002, we also studied the kinetics of the circulating dendritic cell subset during G-CSF mobilization, and we found that the number of lin(-)HLA-DR(+)CD123(+) cells increased while the number of lin(-)HLA-Dr(+)CD11c(+) decreased during G-CSF mobilization, which suggested that G-CSF induced the DC2 dominant state. Further, we found that this DC2 dominant state can be conversed with IL-2 in vitro. These findings suggest that dendritic cell immunotherapy is possible for patients with childhood cancer following autologous peripheral blood stem cell transplantation, and the modulation of the apheresed products may change the outcome of stem cell transplantation including treatment of graft versus host disease (GVHD).

我们研究了盛宴细胞免疫疗法外周血干细胞patiel pail patiel patiel dhooood癌，我们将通过与杂菌（IL） - 3，巨噬细胞（IL），巨噬细胞（IL），巨噬细胞（IL），巨核（IL），巨核（IL），摩托果皮（IL），从融化的周围流血的CD34^+细胞分数中获得功能性的树突状细胞。菌落刺激剂（GM-CSF）和肿瘤坏死因子（TNF）-α，以及从2001年用IL-4和GM-CSF培养的CD14^+细胞。脐带血细胞单位E，我们能够使用Mini MACS分离试剂盒将树突状细胞与用于用G-CSF动员的花序产物分离出来。 CSF动员帽LIN（ - ）HLA-DR（+）CD123（+）细胞的数量增加，而LIN（ - ）CD11C（+）的数量在G-CSF动员过程中减少了，这表明G-CSF诱导了主要的状态。我们发现，这种DC2主导状态可以在体外使用IL-2转化。 ）。

项目成果

渡辺 力: "末梢血幹細胞移植の実際"原田実根, 園田精昭, 高上洋一編;南光堂. 4 (2001)

Tsutomu Watanabe：《外周血干细胞移植实践》，Mine Harada、Seiaki Sonoda、Yoichi Takagami 4 (2001)。

Watanabe T et al.: "HLA-identical sibling peripheral blood stem cell transplantation in children and adolescents"Biology of Blood and Marrow Transplantation. 8. 26-31 (2002)

Watanabe T 等人：“儿童和青少年的 HLA 相同同胞外周血干细胞移植”血液和骨髓移植生物学。

Watanabe T et al.: "Effect of granulocyte colony-stimulating factor on bone metabolism during peripheral blood stem cell mobilization."International Journal of Hematology. 77. 75-81 (2003)

Watanabe T 等人：“粒细胞集落刺激因子对外周血干细胞动员过程中骨代谢的影响。”国际血液学杂志。

Watanabe T et al.: "Effect of granulocyte colony-stimulating factor on bone metabolism during peripheral blood stem cell mobilization"International Journal of Hematology. 77. 75-81 (2003)

Watanabe T 等人：“粒细胞集落刺激因子对外周血干细胞动员过程中骨代谢的影响”国际血液学杂志。

Watanabe T et al.: "HLA-identical sibling peripheral blood stem cell transplantation in children and adolescents"Biology of Blood and Marrow Transplantation. 8. 26-31 (2001)

Watanabe T 等人：“儿童和青少年的 HLA 相同同胞外周血干细胞移植”血液和骨髓移植生物学。