Investigation of Basal Ganglia Function and Intrinsic Dopamine Release by Positron Emission Tomography

正电子发射断层扫描研究基底神经节功能和内在多巴胺释放

• 批准号: 12670635
• 负责人: ISHII Kenji
• 金额: $ 2.05万
• 依托单位: Tokyo Metropolitan Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670635/
• 关键词: PET; dopamine; raclopride; basal ganglia; voluntary movement; learning; human; brain; ポジトロンCT; ドーパミン放出; 書字; 脳賦活検査; ドーパミン受容体

To investigate the role of nigrostriatal dopamine system in performing a skilled complex motor task without leaning such as writing, we measured endogenous dopamine release in human brain with ^<13>C-raclopride (RAC) and PET.We studied six right-handed healthy volunteers (ages 22.0±1.7 years). After an intravenous injection of RAC, a 60 minites dynamic scan was performed. Each subject participated in RAC-PET twice on the same day (scan interval 145.0±35.2 minutes) under two different conditions ; during hand writing and at rest in balanced order. In the writing condition, the subjects was asked to dictate sentences as quick as possible on a computer-controlled writing pad with pen tool using their right hand. In resting condition, the subject was asked to watch the display without any movement. Endgeneous dopamine release was estimated indirectly based on the occupancy model, from the difference of the binding potential (BP) of PAC in two different conditions. The same subjects also participated in H_<2>^<15>O-PET with the same paradigm to evaluate the regional cerebral activation with the task in terms of regional cerebral blood flow change.The left striatum was activated significantly with H_<2>^<15>5O-PET study with writing task. In the same region, the binding potentials of RAC was significantly reduced by dictation task, thus, the intrinsic dopamine release was considered to be accelerated by the task. The activated region located in the ventro-caudal portion of the putamen which we previosly reported to be corresponding to hand area in the sometotopic organization in the basal ganglia. This result demonstrated the ability of indirect measurement method of intrinsic dopamine release by RAC-PET in physiological condition. Our results are the first report of activation of dopaminergic neurons by a skilled voluntary monements.

为了调查黑质多巴胺系统在执行熟练的复杂运动任务中的作用而不依赖诸如书写，我们测量了 ^<13> c-raclopride（RAC）和PET的人脑中内源性多巴胺释放。静脉注射RAC后，进行了60分钟的动态扫描。每个受试者在两个不同的条件下在同一天两次参加RAC-PET（扫描间隔145.0±35.2分钟）；在手写期间，按平衡顺序进行休息。在写作条件下，要求受试者用右手使用笔工具在计算机控制的写作板上尽快指示句子。在休息状态下，要求受试者观看显示器而没有任何动作。根据两种不同条件下PAC的结合势（BP）的差异，根据占用模型间接估计多巴胺释放。同一受试者还参加了具有相同范式的H_ <2>^<15> O-PET，以通过局部脑血流变化来评估该任务的区域大脑激活。用H_ <2>^>^<2>^<15> 5o-pet研究通过写作任务显着激活了左纹状体。在同一区域中，RAC的结合势被命令任务显着降低，因此，该任务认为内在的多巴胺释放被认为是加速的。激活的区域位于鬼盘的Ventro-auaudal部分，我们以前据报道与巴萨神经节的某个动物组织中的手区域相对应。该结果证明了RAC-PET在物理条件下固有多巴胺释放的间接测量方法的能力。我们的结果是通过熟练的自愿性摩托车激活多巴胺能神经元的首次报道。

项目成果

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Nariai, T., Ohno, K., Ohta, Y., Hirakawa, K., Ishii, K. and Senda, M.: "Discordance between cerebral oxygen and glucose metabolism, and hemodynamics in a mitochondrial encephalomyopathy, lactic acidosis, and strokelike episode.patient"J Neuroimag,. 11(3).

Nariai, T.、Ohno, K.、Ohta, Y.、Hirakawa, K.、Ishii, K. 和 Senda, M.：“线粒体脑肌病、乳酸性酸中毒和脑氧代谢和葡萄糖代谢与血流动力学之间的不一致

Suzuki, Y., Kiyosawa, M., Mochizuki, M., Ishii, K. and Senda, M.: "Cortical blindness following aortic arch surgery"Jpn J Ophthalmol. 45. 547-549 (2001)

Suzuki, Y.、Kiyosawa, M.、Mochizuki, M.、Ishii, K. 和 Senda, M.：“主动脉弓手术后皮质失明”Jpn J Ophamol。

Mishina, M. et al.: "Intrasubject correlation between static scan and distribution volume images for [C] flumazenil PET"Ann Nuol Med. 14. 193-198 (2000)

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Chno T,Band,M,Nagura H Ishii K,Yamanouch H: "Apraxic agraphia due to thalamic infaction"Neurology. 54. 2336-2339 (2000)

Chno T，Band，M，Nagura H Ishii K，Yamanouch H：“丘脑梗塞引起的失语症”神经病学。