Human T-Cell Leukemia Virus Type I Tax Protein Induces the Expression of IL-6R and soluble IL-6R production

人 T 细胞白血病病毒 I 型 Tax 蛋白诱导 IL-6R 表达和可溶性 IL-6R 产生

• 批准号: 12670275
• 负责人: HORIUCHI Sankichi
• 金额: $ 1.22万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670275/
• 关键词: HTLV-1; IL-6R; Soluble IL-6R; ATL; HAM; IL-6; Jak; STAT; 成人T細胞白血病; HTLV-I関連脊髄麻痺

Aim : The soluble interleukin 6 receptor (sIL-6R) circulates at elevated levels in HTLV-I infected patients compared with the healthy person. To elucidate the cause of this high level release of SIL-6R in HTLV-I infected patients, we investigated expression of the IL-6R or sIL-6R in T-cells infected with HTLV-I. Results and DiscussionrThrough our examination, it was found that although some HTLV-I-non infected cell lines expressed IL-6R, all HTLV-I-infected cell lines tested expressed IL-6R.Results and Discussion : Through our examination, it was found that although some HTLV-I-non-infected cell lines expressed IL-6R, all HTLV-I-infected cell lines tested expressed IL-6R, Although the TY8-3 T-cell established from a thymoma did not express IL-6R, the HTLV-I infected isogenic cell lines, TY8-3/MT-2 and TY8-3/TCL-Kan expressed IL-6R. The release of proteolytically cleaved (PC)-sIL-6R was demonstrated on MT-2, MT-4, TY8-3/MT-2 and TY8 3/TCL-Kan, but not HTLV-I negative parental TY8-3 by ELISA. Induction of the Tax in JPX-9 cells by CdCl_2 results in the expression of not only IL-6R but also release of the PC-IL-6R, but not in JPX-9/M. Our results demonstrate that expression of the IL-6R and release of the PC-'sIL 6R are induced by HTLV-I or Tax. Hence, it may contribute to affected tissues, in HTLV-I associated inflammatory diseases, because the presence of sIL-6R is central to the regulation of IL-6-mediated responses in signaling for inflammatory cytokine IL-6 in the cells which are not expressed the cognate IL-6R.

目的：与健康人相比，HTLV-1感染患者的可溶性白介素6受体（SIL-6R）在升高的水平下循环。为了阐明在HTLV-I感染患者中SIL-6R的高水平释放的原因，我们研究了感染HTLV-I的T细胞中IL-6R或SIL-6R的表达。结果和讨论通过我们的检查，发现尽管某些HTLV-I-non感染的细胞系表示IL-6R，但所有HTLV-I感染的细胞系测试均表达IL-6R. Results和讨论：通过我们的检查：发现它，发现尽管某些HTLV-I未感染的细胞系表示IL-6R，但所有htlv-i感染的细胞系都测试了表达IL-6R的细胞系，尽管从胸腺瘤建立的TY8-3 T细胞均未表达IL-6R，但HTLV-I感染的ISEGENIC细胞系TY8-3/MT-2和TY8-3/TCL-KAN表达IL-6R。 ELISA在MT-2，MT-4，TY8-3/MT-2和TY8 3/TCL-KAN上释放（PC）-SIL-6R在MT-2，MT-4，TY8-3/MT-2和TY8 3/TCL-KAN上证明了ELISA的HTLV-I负亲属TY8-3 。通过CDCL_2在JPX-9细胞中诱导税收不仅会导致IL-6R的表达，还会释放PC-IL-6R，而在JPX-9/m中则释放。我们的结果表明，IL-6R的表达和PC-'SIL 6R的释放是由HTLV-I或税收诱导的。因此，在HTLV-I相关炎性疾病中，它可能有助于受影响的组织，因为SIL-6R的存在对于不属于不属于的炎性细胞因子IL-6的信号传导调节IL-6介导的反应至关重要。表示同源IL-6R。