A study on the inhibitory action of vitronnectin on staphylococcal leukocidin and gamma-hemolysin

玻连蛋白对葡萄球菌杀白细胞素和γ-溶血素抑制作用的研究

• 批准号: 12670246
• 负责人: TOMITA Toshio
• 金额: $ 2.24万
• 依托单位: Graduate School of Agricultural Science, Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670246/
• 关键词: Staphylococcus aureus; bi-component cytolysins; staphylococcal gamma-hemolysin; staphylococcal leukocidin; serum inhibitor; vitornectin; extracellular matrix

Staphylococcal γ-hemolysin and leukocidin are bi-component cytolysins, consisting of LukF (or Hlg1)/Hlg2 and LukF/LukS, respectively. We have previously purified a serum inhibitor of γ-hemolysin and leukocidin, and identified it as vitronectin. In this study, we showed that (i) vitronectin fragments specifically bound to the Hlg2 component of γ-hemolysin and the LukS component of leukocidin to prevent the toxin-induced lysis of human erythrocytes and human polymorphonuclear leukocytes, respectively, (ii) vitronectin fragments and Hlg2 component (or LukS component) formed high-molecular-weight complexes that co-sedimented in a sucrose gradient centrifugation and co-migrated on a native polyacrylamide gel electrophoresis, (iii) the co-sedimented complexes contained vitronectin and the toxin component in an approximate molar ratio of 6 : 1, and (iv) the complexes had spherical shape with diameter of approximately 20 nm. The ability of γ-hemolysin and leukocidin to bind vitronectin is a novel function of the pore-forming cytolysins.

葡萄球菌γ-溶血素和白细胞素A BI组分，由LUKF（或HLG1）/HLG2和LUKF/LUKs组成，我们已经纯化了γ-蛋白酶素和白细胞素的血清抑制剂，并将其鉴定为Vitronectin。结果表明，（i）特异性与γ-蛋白的HLG2成分和白细胞素的luks成分结合到毒素诱导的人类红细胞和人类多形核核细胞裂解的裂解，（ii）VITRONECTIN片段和HLG2组成部分（或HLG2组成部分）（或HLG2组成部分）组件）形成的高分子量复合物，在蔗糖梯度集中术中共同使用，并共同迁移了天然的poleacrylamide凝胶电泳，共二二聚体的COMP络合物包含玻璃体蛋白和毒素成分，在6：1，6：1，Appox Molar比率中（iv）复合物的螺旋形状约为20 nm。

项目成果

H.Sawada, Y.Mural, M.Kurachi, T.Kawase, Y.Minami, J.Kyokane, J., T.Tomia: "Synthesis and antibacterial ativity of novel fluoroalkyl end-capped oligomers containing ammonium segments : application to new fluorinated gelling materials with an antibacterial"

H.Sawada、Y.Mural、M.Kurachi、T.Kawase、Y.Minami、J.Kyokane、J.、T.Tomia：“含铵链段的新型氟烷基封端低聚物的合成和抗菌性：在新材料中的应用

H.Sawada, K.Yanagida, Y Inaba, M.Sugiya, T.Kawase, T.Tomita: "Synthesis and antibacterial activity of novel fluoroalkyl end-capped cooligomers containing dimethyl(octyl)ammonium segments"Eur.Polym.J.. 37(7). 1433-1439 (2001)

H.Sawada、K.Yanagida、Y Inaba、M.Sugiya、T.Kawase、T.Tomita：“含有二甲基（辛基）铵片段的新型氟烷基封端低聚物的合成和抗菌活性”Eur.Polym.J..

A.H.Nguyen, T.Tomaita, M.Hirota, J.Kaneko, T.Hayashi, Y.Kamio: "DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of Erwinta carotovora subsop.carotovora Er"J.Bacteriol. 183(21). 6274

A.H.Nguyen、T.Tomaita、M.Hirota、J.Kaneko、T.Hayashi、Y.Kamio：“尾部纤维基因中的 DNA 倒置改变了胡萝卜素 Er 的宿主范围特异性，胡萝卜素 Erwinta 胡萝卜素是一种噬菌体尾巴样细菌素

Y.Kamio, T.Tomita, J.Kaneko: "Kluwer Academic/Plenum Publishers, New, York"Pore-forming cytolysin from Stapylococcus aureus, α-and γ-hemolysin, and leukocidin, in Staphylococcal Infection and Immunity, ed. by I. Friedman. 330 (2001)

Y. Kamio、T. Tomita、J. Kaneko：“Kluwer Academy/Plenum Publishers，纽约州”金黄色葡萄球菌的成孔溶细胞素、α-和 γ-溶血素以及杀白细胞素，《葡萄球菌感染与免疫》，编辑。 I.弗里德曼。330（2001）

Nakamura, K., Tomita, T., Abe, N., Kamio, Y.: "Purification and characterization of an extracellular poly (L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K 104-1"Appl. Envirn. Microbiol.. 67 (11). 345-353 (2001)

Nakamura, K.、Tomita, T.、Abe, N.、Kamio, Y.：“来自土壤分离物 Amycolatopsis sp. K 104-1 菌株的细胞外聚（L-乳酸）解聚酶的纯化和表征”应用