Morphological variation and cell adhesion of terminal Schwann cells-- a study by three-dimensional microanatomy and immunohistochemistry.

终末雪旺细胞的形态变异与细胞粘附——三维显微解剖和免疫组化研究。

基本信息

批准号：
12670001
负责人：
IWANAGA Hiromi
金额：
$ 1.98万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Morphology and development of terminal Schwann cells associated with various sensory endings were analyzed both by three-dimensional microanatomy based on combined observation by scanning and electron microscopy, and by immunohistochemistry utilizing an antibody against the Schwann cell marker, S100β protein. Comparative observation of periodontal Ruffini endings and vibrissal lanceolate endings in rats and Meissner corpuscles in monkey palmar skn showed that terminal Schwann cells in these mechanoreceptors consistently issued main, lamellar processes to envelope axon endings. The main processes were provided with fine accessory projections specific to receptor types in morphology. The accessory projections of the terminal Schwann cells linked the different mechanoreceptors with surrounding tissues in distinct manners, suggesting that tissue deformations induced by external forces are transmitted to receptor sites of the axon terminals via the fine anchors. Maturation of terminal Schwann cells was observed in vibrissal lanceolate endings of 2 to 3 week-old rats. Immature Schwann cells, which were stellate in shape with fine processes radiating from the cell body, occurred in connective tissue surrounding lanceolate endings. These cells were gradually transformed into terminal Schwann cells, extending main processes along axon terminals. In the process of maturation, the great majority of the fine radial processes of the Schwann cels regressed, while the remainders became the anchoring, accessory projections. These findings indicate that the morphogenesis of terminal Schwann cells is induced by contact with axon terminals. Signaling substances and cell adhesion molecules involved in the cell interaction remain to be elucidated.

基于扫描和电子显微镜的组合观察以及通过针对Schwann细胞标记的S100β蛋白的抗体，通过扫描和电子显微镜组合进行了三维微型分析，分析了与各种感觉末端相关的末端Schwann细胞的形态和开发。牙周r子末端的比较观察和猴子棕榈Skn中的Meissner甲状腺的纤维状披针形末端表明，这些机制感受器中的末端schwann细胞始终发出主要的层状过程，以构造轴突末端。主要过程提供了特定于受体类型的出色附件项目。末端Schwann细胞的辅助项目以不同的方式将不同的机制与周围的时间联系起来，这表明外力诱导的组织变形通过细锚传递到轴突末端的受体位点。在2至3周大的大鼠的振动披针形末端观察到末端雪旺氏细胞的成熟。未成熟的Schwann细胞在形状上是星体的精细过程，从细胞体辐射出来，发生在披针形末端周围的结缔组织中。这些细胞逐渐转化为末端雪旺细胞，沿轴突末端扩展了主要过程。在成熟过程中，Schwann Cels的大部分精细径向过程都退缩了，而其余的则成为锚定的附属项目。这些发现表明，末端雪旺细胞的形态发生是与轴突末端接触的。涉及细胞相互作用的信号传导物质和细胞粘附分子仍有待阐明。