Development of a novel enzymatic synthesis method for trehalose derivatives and its application

海藻糖衍生物酶法合成新方法的建立及其应用

基本信息

批准号：
12839003
负责人：
SODE Koji
金额：
$ 2.18万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12839003/
关键词：
trehalose trehalase enzyme inhibitor glucose-3-dehydrogenase insecticide Halomonas sp.

项目摘要

We aimed at the development of novel enzymatic synthesis method of the trehalose derivatives and those applications to insecticides or anti-fungal chemicals. Trehalose is a disaccharide and commonly found in fungi, algae and in several invertebrate phyla but not in flowering plants and vertebrates. It presents in all insects and in many insects it is present in high concetration s and constitutes the major hemolymph sugar. Trehalose secifically hydrolyzed trehalose and is the rate-limiting enzyme in the process of glycogen synthesis from hemolymph trehalose in developing ovaries of the silkworm. Therefore the specific inhibitor for trehalases is a candidate of novel insecticides.We purified glucose-3-dehydrogenase from Halomonas sp. A -15 and found its activity to produce trehalose derivatives. One of those derivatives, 3,3'-diketotrehalose showed an inhibitory activity to trehalose, and therefore we investigated its activity as insecticide or anti-fungal chemicals. It did not show anti-fungal activity but it can be an insecticide. We also investigated its efficient production process, and found the enzymatic production is more efficient than microbial production. By using potassium ferricyanide as electron acceptor and the highly purified glucose-3-dehydrogenase, 80% productivity was attained. Hence we established efficient method to produce 3,3'-diketotrehalose, it can contribute as a novel effective insecticide which is not harmful to animals.

我们旨在开发海藻糖衍生物的新型酶合成方法，以及这些应用于杀虫剂或抗真菌化学物质。海藻糖是一种二糖，通常在真菌，藻类和几个无脊椎动物门中发现，但在开花植物和脊椎动物中没有发现。它呈现在所有昆虫中，在许多昆虫中都存在于高处，并构成主要的血淋巴糖。海藻糖依赖性水解海藻糖，是速率限制酶从囊虫的卵巢中从糖原合成的过程中。因此，trehalases的特异性抑制剂是新型杀虫剂的候选。 A -15并发现其活性产生海藻糖衍生物。其中一种衍生物之一，3,3'-二甲透析糖表现出对海藻糖的抑制活性，因此我们研究了其作为杀虫剂或抗真菌化学物质的活性。它没有显示抗真菌活性，但可以是一种杀虫剂。我们还研究了其有效的生产过程，并发现酶促产生比微生物生产更有效。通过使用铁氰化钾作为电子受体和高度纯化的葡萄糖-3-脱水酶，可以达到80％的生产率。因此，我们建立了有效的方法来产生3,3'-二甲状腺解读，它可以作为一种新的有效杀虫剂，对动物无害。