Fiber-optic approaches for diagnosis of gastrointestinal disease

诊断胃肠道疾病的光纤方法

• 批准号: 12359001
• 负责人: SHIMOSEGAWA Tooru
• 金额: $ 26.35万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12359001/
• 关键词: photodynamic therapy; mono-l-aspartyl chlorin e6(Npe 6); raman specrtoscopy; Ti:sapphire laser; optical biopsy; fiber optic probe; ラマン散乱; 波長可変レーザー; 生体分光; 光ファイバー; 消化器腫瘍

To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6(NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model. (1) The intestinal tumor sites were irradiated with a 664-nm diode laser constant intervals after the administration of photosensitizers, respectively. (2) The tumors were excised and processed for the frozen section to observe the fluorescence by a microscope. We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (P<0.001). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image. We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.Our major goal is to develop advanced analytical techniques for biomedical researches based on the Raman spectroscopic applications of an electronically tuned Ti:sapphire laser and the developments of advanced spectroscopic tools.

为了阐明第二代光敏剂单-L-天冬氨酰二氢卟酚e6（NPe6）的有用性，我们在小鼠模型中研究了光动力诊断和光动力治疗肠道肿瘤的可能性。 (1)给予光敏剂后，分别用664nm二极管激光恒定间隔照射肠肿瘤部位。 (2)切除肿瘤，制作冰冻切片，显微镜下观察荧光。我们观察荧光图像并计算6小时内肿瘤中的平均荧光强度显着高于正常粘膜（P<0.001）。 NPe6的荧光主要积聚在肠肿瘤中，在荧光显微照片上呈红色荧光。我们的结论是，NPe6 可能是一种有价值的光敏剂，可用于肠道肿瘤的光动力诊断和光动力治疗。我们的主要目标是基于电子调谐钛宝石激光器的拉曼光谱应用以及先进的光谱工具。

项目成果

Qualitative analysis of trace Raman bands of non-resonant species in near-infrared excited pre-resonance Raman spectra of hemoglobin by partial least square regression-Raman excitation profile method

偏最小二乘回归-拉曼激发剖面法定性分析血红蛋白近红外激发预共振拉曼光谱中非共振物质的痕量拉曼带

• 发表时间: 2004
• 期刊: Vibrational Spectroscopy 34
• 作者: Sato H;Wada S;et al.
• 通讯作者: et al.

動脈硬化症診断のための分光分析技術

动脉硬化诊断的光谱分析技术

• 发表时间: 2004
• 期刊: レーザー医学会誌 25
• 作者: 佐藤英俊;小町裕一;朝倉徹;下瀬川徹;會沢勝夫;田代英夫
• 通讯作者: 田代英夫

Phetodynamic therapy of intestinal tumors with mono-L-Aspartyl chlorin e6 (Npe6): a basic study

单-L-天冬氨酰氯 e6 (Npe6) 光动力疗法治疗肠道肿瘤：一项基础研究

• 发表时间: 2003
• 期刊: Anticancer Reserch 23
• 作者: Kikuchi T;Asakura T;et al.
• 通讯作者: et al.

Fluorescence background less Ti:sapphire laser using acoust-optical tunable filter for Raman spectroscopic measurements

使用声光可调谐滤波器进行拉曼光谱测量的无荧光背景钛宝石激光器

• 发表时间: 2002
• 期刊: Appl Spectrosc 56
• 作者: H.Sato;S.Wada;H.Tashiro
• 通讯作者: H.Tashiro

Sato H, Saito K, et al.: "Electronically tunable-laser light sources for NIR spectroscopy"J.Near Infrared Spectroscopy. 11. 295-308 (2003)

Sato H、Saito K 等人：“用于近红外光谱的电子可调激光光源”J.近红外光谱。