Studies on the development of the periodontal examination by using saliva

唾液牙周检查技术的发展研究

• 批准号: 12357014
• 负责人: KURIHARA Hidemi
• 金额: $ 27.56万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12357014/
• 关键词: saliva; periodontal examination; outer membrane protein; Streptococcus anginosus; diabetes; matrix metalloproteinase-8; 8-hydroxy-deoxyguanosine; sulfated glycosaminoglycan; ストレプトコッカス アンギノサス; 硫酸化グコサミノグルカン; Porphyromonas gingivalis; Actinobacill

In this project, we analyzed the development of the periodontal examination by using saliva from the diversified aspects. There were products of IgA antibody against Porphyromonas gingivalis (P.g) in saliva from patients with progressive periodontitis. And it can be possible to identify the P.g infection by measurement of specific IgA antibody in saliva. We demonstrated the detection method of 9 putative periodontopathic bacteria in saliva by polymerase chain reaction (PCR). IgA in saliva from periodontal patients, which serum strongly react outer membrane protein (Omp) from Actiobacillus actiomycetemcomitans (A.a), reacted A.a-Omp antigen in enzyme-linked immunosorbent assay (ELISA). And this method enable us to identify the infection of A.a. We established the specific PCR-detection method of Streptococcus anginosus (S.a), and revealed the unique relationship between S.a and antibacterial peptide (hBD-2) from epitherial cells. And a part of molecular mechanism of S.a pathogenicity wa … More s clear. We found that it was available to use a diabetic patient model, which was improved the insulin resistance and control level of Blood sugar by periodontal treatment, to develop the periodontal examination by using saliva. There was no difference between diabetic group with periodontal disease and that without periodontal disease in the point of biochemical examination data by using saliva, so we could reveal no relevance between diabetes and periodontitis. To examine the elastase activity and the expression of active oxygen in neutrophil from saliva, it was useful to realize the state of periodontal disease and to judge the risk of that. By measuring the level of active form of matrix metalloproteinase-8 (MMP-8) in saliva, we can select the patient with active type periodontitis. The mean value of 8-hydroxy-deoxyguanosine (8-OHdG) in saliva of periodontal patients was higher than that of normal subjects. So 8-OHdG levels in saliva appear to reflect the status of periodontal health. Sulfated glycosaminoglycan (S-GAG), which was indicated as the good marker of alveolar bone metabolism, couldn't detect in saliva from the subjects with gingivitis Less

在这个项目中，我们通过使用牙周炎的患者进行了针对牙周牙龈（P.G）的牙周检查的牙周检查的发展。 。 ，并从epitherial细胞和S.AA致病性WA的分子机制的一部分来使用S.AA和抗菌肽（HBD-2）之间的独特关系，可用于使用糖尿病患者模型通过牙周治疗的耐药性和控制水平，通过使用唾液来发展牙周检查，并在生物化学EXATA的角度使用唾液Ando检查中性氧气和活性氧气中的中性粒细胞的表达。通过测量唾液的活性MMP-8的水平，我们可以在唾液中选择牙周疾病。患者高于正常受试者。

项目成果

Nomura, Y.: "Mutans streptococci and Lactobacillus as risk factors for dental caries in 12-year-old children"Jpn J Infect Dis. 54. 43-45 (2001)

Nomura, Y.：“变形链球菌和乳酸菌是 12 岁儿童龋齿的危险因素”Jpn J Infect Dis。

Senpuku, H.: "Inhibitory effects of MoAbs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of Streptococcus mutans"Scand J Immunol. 54. 109-116 (2001)

Senpuku, H.：“MoAb 对表面蛋白抗原在变形链球菌体外实时粘附和体内再定殖中的抑制作用”Scand J 免疫学。

Takeuchi, Y.: "Treponema socranskii, Treponema denticola, and Porphyromonas gingivalis are associated with severity of periodontal tissue destruction"J Periodontol. 72. 1354-1363 (2001)

Takeuchi, Y.：“索氏密螺旋体、齿状密螺旋体和牙龈卟啉单胞菌与牙周组织破坏的严重程度有关”《牙周病杂志》。

Sakamoto, M. et al.: "Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA Libraries"Microbiol Immunol. 44. 643-652 (2000)

Sakamoto, M. 等人：“通过 16S rDNA 文库序列分析比较健康受试者和两名牙周炎患者唾液中的口腔细菌菌群”Microbiol Immunol。

Sakamoto, M.: "Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA Libraries"Microbiol Immunol. 44. 643-652 (2000)

Sakamoto, M.：“通过 16S rDNA 文库序列分析比较健康受试者和两名牙周炎患者唾液中的口腔细菌菌群”Microbiol Immunol。