Exploration of new genes related to achivement of transplantation tolerance in living donor liver transplantation

活体肝移植中与移植耐受实现相关的新基因探索

基本信息

批准号：
12307024
负责人：
UEMOTO Shinji
金额：
$ 21.58万
依托单位：
Mie University (2001-2002)Kyoto University (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307024/
关键词：
gene DANmicroarray living donor liver transplantation Immunosuppression transplant tolerance

项目摘要

Seventy recipients were enrolled in elective weaning immunosuppression at Kyoto University Hospital. Twenty-four recipients achieved complete withdrawal of immunosuppression, 37 were during weaning, and 9 encountered rejection. Fifty-four recipients were enrolled in nonelective weaning of immunosuppression. Thirty-four recipients achieved complete withdrawal, 7 were during weaning, and 13 encountered rejection. At Mie University Hospital, 3 recipients were enrolled in nonelective weaning because of EB virus infection. One recipient achieved complete withdrawal, but remaining 2 encountered rejection.Total RNA was extracted from peripheral blood lymphocytes of recipients who accomplished complete withdrawal of immunosuppression and normal volunteers. RNA was analyzed by cDNA microarray (Agilent) comparing mRNA expression of recipients with that of normal volunteers. Number of genes with up-regulated mRNA expression was 120, and that with down-regulated expression was 169. Genes with up-regulated or down-regulated expression were divided into subgroups according to gene-related function. Number of up-regulated or down-regulated genes were 4/3 for [Apoptosis], 2/2 for [Cell cycle], 2/7 for [Cytokine], 4/4 for [DNA metabolism], 1/0 for [Heat shock protein], 3/7 for [Receptor], 16/18 for [Signal transduction], 7/14 for [Stress], 8/13 for [Transcription], 9/14 for [Transporter], and 72/113 for undetermined factor, respectively.

在京都大学医院，有70名接受者参加了选择性断奶免疫抑制。二十四名接受者完全戒断了免疫抑制，有37名在断奶期间，有9名遭到拒绝。五十四名接受者参加了免疫抑制的非选择性断奶。 34名接收者完全戒断，有7名在断奶期间，有13人遇到拒绝。在MIE大学医院，由于EB病毒感染，有3名接受者入学了非选择性断奶。一名接受者完全戒断，但剩下的2个遇到的排斥反应是从接受免疫抑制和正常志愿者完全戒断的受体的外周血淋巴细胞中提取的。通过将受体的mRNA表达与正常志愿者的mRNA表达进行比较，对RNA进行了分析。具有上调mRNA表达的基因的数量为120，表达下调的基因为169。根据基因相关功能将具有上调或下调表达的基因分为亚组。 [凋亡]的上调或下调基因的数量为4/3，[细胞周期] 2/2，[细胞因子] 2/7，[DNA代谢]为4/4，[DNA代谢]，[热休克蛋白]的1/0，[受体]的3/7，[受体]的3/7，16/18，用于[信号转移]的16/14 for [信号转移]，7/1/1/1/1/1/1/1/1/1/13 [转运蛋白]和72/113分别为未确定的因子。