Drug Delivery System of Insulin and Calcitonin through the Scrotum of Rats.

胰岛素和降钙素通过大鼠阴囊的药物输送系统。

• 批准号: 62570963
• 负责人: OKUMURA Katsuhiko
• 金额: $ 1.15万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570963/
• 关键词: Scrotum; Percutaneous Absorption; Insulin; Cyclosporin A; Drug Delivery System(DDS); Corneum; Peptide; 角質層; ドラッグデリバリーシステム

In order to clarify the absorption mechanism of peptide drugs through the scrotum, pharmaceutical, pharmacological, and histological studies were performed. Insulin and cycolsporin A were selected as model peptide, and following results were obtained.1. Absorption of Insulin through the Scrotum: (1) Insuliin was hardly absorbed through the sctotum of rats when no enhancer was added in insulin solution. (2) The absorption of insulin throgh the scrotum was greatly enhanced by the addition of azone and salicylic acid which remove the corneum of scrotum. the removal of corneum with adhesive tape also enahanced the insulin absorption, indicating importance of corneum as a transport barrier. (3) The enhanced absorption was abserved when iontophoresis was used, however the skin irritation and ski damage were observed simultaneously.2. Absorption of Cyclosporin A through the Scrotum: (1) The absorption of cyclosporin A was enhanced by the concomitant administration of azone and dimethylsulfoxide, though its absourption was limited without any enhancer. (2) The histological obsevation indicated the correlation between absorption and thickness of corneum.

为了阐明肽类药物通过阴囊的吸收机制，进行了药学、药理和组织学研究。选择胰岛素和环孢素A作为模型肽，得到以下结果： 1．胰岛素经阴囊吸收：(1)胰岛素溶液中不添加促进剂时，大鼠阴囊几乎不吸收胰岛素。 (2)加入氮酮和水杨酸，去除阴囊角质，大大增强了阴囊对胰岛素的吸收。用胶带去除角质层也增强了胰岛素的吸收，表明角质层作为运输屏障的重要性。 (3)离子电渗疗法吸收增强，但同时出现皮肤刺激和滑雪损伤。2．环孢菌素A通过阴囊的吸收：(1)同时给予氮酮和二甲基亚砜可增强环孢菌素A的吸收，但在没有任何增强剂的情况下其吸收受到限制。 (2)组织学观察表明吸收与角质层厚度之间存在相关性。

项目成果

K.Okumura,;M.Ando,;R.Hori.: J.Phewm.Sci.

K.Okumura,;M.Ando,;R.Hori.：J.Phewm.Sci。

K. Okumura; M. Ando; R. Hori: "Absorption of Cyclosporin A through the Scrotum in Rats." J. Pharm. Sci.

K.奥村；

R. Hori;K. Okumura;M. Ando: Pharmaceutical Rescarch.

R.堀；K.