Chromosome constitution of gastric and colon cancer and the relevance to cancer progression.

胃癌和结肠癌的染色体构成及其与癌症进展的相关性。

基本信息

批准号：
62570333
负责人：
MISAWA Shinichi
金额：
$ 1.28万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570333/
关键词：
Chromosomes Gastric cancer Ascites Human Chromosomes Human Gastric cancer cancerous effusion 構造異常大腸癌

项目摘要

Chromosome constitution was studied in 11 patients (4 males and 7 females, age at 34 to 69 years) using cancerous effusions (ascites in 10 patients and pleural effusion in 1). Macroscopic diagnosis of cancer was IIc+III (advanced) in 2 patients, Borr. 2 type in 1 , Borr. 3 in 1 , and Borr. 4 in 7. Pathological diagnosis was tubular adenocarcinoma in 1 patient, poorly differentiated adenocarcinoma in 6, and signet-ring cell carcinoma in 1. Pathological diagnosis was not obtained in the remaining patients after repeated biopsies. Cancerous effusions were cytologically positive except for 1 sample. Cell pellets were cultured for 2 to 24 hours and chromosomes were analyzed after standard G-banding methods. Among 11 cases, 9 cases provided abnormal karyotypes and 1 a normal karyotype. The modal chromosome number distributed from diploid to hypotetraploid range. Among numerical and structural aberrations detected, gains of chromosome 7 was seen in 5 patient, chromosomes 2, 13, 20 in 4. Structural changes involving chromosomes X, 1, 3, 5, 12, 17 were recurrently observed, which resulted in deletions of Xq, 3q, 5q, 6q or chromosome involvements of lp36, 9p2l-24, 12ql2-13, 17cen-pll.2. Some of these abnormalities may have relevance to the carcinogenesis or progression of gastric cancer.

利用癌性积液（10 例腹水、1 例胸腔积液）对 11 名患者（4 名男性和 7 名女性，年龄 34 至 69 岁）的染色体构成进行了研究。 2 名患者的宏观癌症诊断为 IIc+III（晚期），Borr。 2 输入 1 ，Borr。 3 合 1 和 Borr。 7例中有4例，病理诊断为管状腺癌1例，低分化腺癌6例，印戒细胞癌1例。其余患者多次活检后未得到病理诊断。除 1 个样本外，癌性渗出液细胞学呈阳性。将细胞沉淀培养 2 至 24 小时，并按照标准 G 带方法分析染色体。 11例中，9例核型异常，1例核型正常。众数染色体数目分布在二倍体到亚四倍体范围内。在检测到的数量和结构畸变中，5 名患者出现 7 号染色体增加，4 名患者出现 2、13、20 号染色体增加。反复观察到涉及 X、1、3、5、12、17 号染色体的结构变化，导致Xq、3q、5q、6q 或 lp36、9p2l-24、 12ql2-13、17cen-pll.2。其中一些异常可能与胃癌的发生或进展有关。