Dynamics of enteropathogenic bacteria in Asian environment

亚洲环境中肠道致病菌的动态

基本信息

批准号：
11691207
负责人：
NISHIBUCHI Mitsuaki
金额：
$ 7.06万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11691207/
关键词：
Asia Aquatic environment Seafood Vibrio parahaemolyticus Escherichia coli O157 Vibrio cholerae

项目摘要

We reported frequent infection due to a new clone of Vibrio parahaemolyticus in eight countries and the United States since 1996. It allowed us to use the term "pandemic." We initially defined the pandemic clone by its O3:K6 serovar, but we subsequently established a toxR-targeted PCR method to identify the pandemic clone. Recently, similar to the O3:K6 pandemic clone, serovariants of the pandemic clone have been spreading across the international border. We have been isolating the pandemic strains, including an O1:K25 strain, from the bivalves in southern Thailand since 1998, and we demonstrated strong association between environmental and clinical strains. The results indicated that improperly cooked bivalves are the means of transmission, and it may be the cause of international spread. Economic factor, ability to purchase expensive seafood, was a more important factor of infection than the poor hygienic condition in Vietnam.We demonstrated that first seafood marketed in Malaysia is frequently contaminated with O139 Vibrio cholerae, which indicates that Malaysian environment is contaminated with this microorganism. We also showed an epidemic due to tetracycline-resistant O1 V. cholerae for the first time in southern Thailand.These results suggest human movement in the southern Thailand-Malaysian region needs to be monitored.We reported contamination of the beef imported from India to Malysia and the beef and cattle feces in Thailand by Escherichia coli O157. We also reported distribution of vancomycin-resistant enterococci and Vibrio vulnificus in Malaysian environment.

我们报告说，自1996年以来，由于八个国家和美国的颤动氧疗程的新克隆引起的经常感染。它使我们能够使用“大流行”一词。我们最初通过其O3：K6血清定义了大流行克隆，但随后我们建立了一种符合TOXR靶向的PCR方法来识别大流行克隆。最近，类似于O3：K6大流行克隆，大流行克隆的血清副体已经遍布国际边界。自1998年以来，我们一直从泰国南部的双壳类中分离出大流行菌株，包括O1：K25菌株，我们证明了环境和临床菌株之间的密切关联。结果表明，不当煮熟的双壳类是传播的手段，这可能是国际传播的原因。与越南较差的卫生状况相比，经济因素是购买昂贵的海鲜的能力，是感染的更重要因素。我们证明，在马来西亚销售的第一种海鲜经常被O139弧菌霍乱污染，这表明马来西亚环境被这种微观机构污染了。我们还首次在泰国南部首次表现出由于耐四环素的O1 V.霍乱而引起的流行病。这些结果表明，需要监测泰国泰国南部 - 马来西亚南部地区的人类运动。我们报告说，我们报道了从印度进口的牛肉污染了印度向马里氏菌和泰国牛肉和牛的牛feces牛肉和牛fecechailandia by Escherichia coli coli coli coli oilio157。我们还报道了马来西亚环境中抗性霉素肠球菌和弧菌的分布。