The immunohistochemical analysis in the mouse stroke model.

小鼠卒中模型中的免疫组织化学分析。

• 批准号: 11835049
• 负责人: YAMANO Mariko
• 金额: $ 1.41万
• 依托单位: Osaka Prefecture College of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11835049/
• 关键词: Pyramidal tract lesion; immunohistochemistry; c-fos; GFAP; central nervous system; muscle; 脳卒中; 脳; 脳卒中モデル動物; 神経活性物質; c-fos; CaM Kinase II

(1) We made stroke model of mouse which were received hemi electrically lesion of pyramidal tract and examined the immunohistochemical changes in the central nervous system and muscles in these animals.(2) Only few c-fos immunoreactivity (IR) were occurred in the rest animals but after treadmill running, rich c-fos-IR nuclei were distributed in the CNS.Glial fibrillary acidic protein (GFAP)-IR and CaM kinase II-IR were a few in the CNS both rest and running animals.(3) In the lesioned animals many changes were occurred. Very rich GFAP-IR and CaM kinase II (CaM II)-IR were seen widely in the operated side. After 30min treadmill running, c-fos-IR were not found in the lesion site especially GFAP-IR and CaMII-IR were rich areas at day 4 and day 7 after surgery. These findings suggest that reactive astrocytes may depressed the neuronal activity at early recovery stage. At day 14 to 1 month, c-fos-IR were gradually increased and GFAP were decreased in the lesioned site.(4) In the muscles, few c-fos-IR nuclei were occurred in the palaritic side on the contrary, many c-fos-IR nuclei were occurred in the normal side at day 4 to day 7. These findings suggest that opposite muscles may enlarged compensation to the palaritic side at early recovery stage.

(1)我们制作了受到锥体束半侧电损伤的小鼠中风模型，并检测了这些动物的中枢神经系统和肌肉的免疫组织化学变化。(2)在模型中仅出现很少的c-fos免疫反应性(IR)。休息动物，但跑步机跑步后，中枢神经系统中分布着丰富的c-fos-IR核。神经胶质纤维酸性蛋白（GFAP）-IR和CaM激酶II-IR在中枢神经系统中休息和运动时都有少量分布。 (3)病变动物发生许多变化。术侧广泛可见非常丰富的 GFAP-IR 和 CaM 激酶 II (CaM II)-IR。跑步机跑30min后，病灶处未发现c-fos-IR，尤其是术后第4天和第7天GFAP-IR和CaMII-IR较丰富。这些发现表明，反应性星形胶质细胞可能会抑制早期恢复阶段的神经元活动。第14天～第1个月，病变部位c-fos-IR逐渐增多，GFAP逐渐减少。(4)肌肉中，肩侧c-fos-IR核很少，相反，c-fos-IR核较多。第4天至第7天，正常侧出现-fos-IR核。这些发现表明，在早期恢复阶段，相对的肌肉可能会扩大对腭侧的代偿。

项目成果

Mariko Yamano: "C-fos expression during experimental stroke in the mice."Acta Anatomica Nipponica. VOL.76-1. 88 (2001)

Mariko Yamano：“小鼠实验性中风期间 C-fos 的表达。”Acta Anatomica Nipponica。

Mariko Yamano: "C-fos expression during exercise in the mice II."Acta Anatomica Nipponica. VOL.75-1. 105 (2000)

Mariko Yamano：“小鼠 II 运动期间 C-fos 的表达。”Acta Anatomica Nipponica。

山野眞利子: "錐体路破壊動物の筋および中枢神経系の動態について"Acta Anatomica Nipponica. 76-1. 88 (2001)

Mariko Yamano：“锥体束破坏动物的肌肉和中枢神经系统动力学”Acta Anatomica Nipponica 76-1（2001）。

山野眞利子: "運動とc-fos蛋白の動態についてII"Acta Anatomica Nipponica. 75-1. 105 (2000)

Mariko Yamano：“c-fos 蛋白 II 的运动和动力学”Acta Anatomica Nipponica 75-1 (2000)。