Effects of exercise and cold exposure on branched-chain amino acids catabolism

运动和寒冷暴露对支链氨基酸分解代谢的影响

• 批准号: 11680048
• 负责人: FUJII Hisao
• 金额: $ 0.45万
• 依托单位: Sendai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680048/
• 关键词: Cold exposure; Exercise training; Branched-chain amino acids catabolism; Rat skeletal muscle; Branched-chain α-keto acid dehydrogenase; 分岐鎖α-ケト酸脱水素酵素複合体; 環境温度ストレス; 寒冷環境; 運動; ラット

The stress of cold exposure increases utilization of protein for energy. Exercise training also promotes protein degradation in skeletal muscle and increases its amino acids catabolism. Therefore, protein consumption in cold-condition sports is greater compared to that in comfortable-condition sports, and the important muscles for performance may be damaged. We examined the effects of exercise training and the stress of cold exposure on a branched-chain α-keto acid dehydrogenase (BCKD) activity in rat skeletal muscle, which is the rate-limiting enzyme of branched-chain amino acids (BCAAs) catabolism. Total activity and activity state (actual activity / total activity) of BCKD in rat skeletal muscle were significantly elevated by exercise training in cold exposure for 3 weeks. These results suggest that exercise training in cold exposure for 3 weeks enhances not only BCAAs catabolism under the resting condition but also the capacity for catabolism during exercise in rat skeletal muscle. In addition, cold exposure for 3 weeks significantly inhibited weight gain. But exercise training in cold exposure 3 weeks led to improve the stagnation of weight gain.

寒冷环境下的应激会增加蛋白质对能量的利用，同时也会促进骨骼肌中蛋白质的降解，增加其氨基酸的分解代谢，因此，与舒适条件下的运动相比，寒冷条件下的蛋白质消耗量更大。我们研究了运动训练和寒冷暴露对大鼠骨骼肌中支链 α-酮酸脱氢酶 (BCKD) 活性的影响，BCKD 是运动的限速酶。通过冷暴露运动训练3周，大鼠骨骼肌中支链氨基酸（BCAA）的总活性和活性状态（实际活性/总活性）显着升高。这些结果表明冷暴露运动训练。 3周不仅增强了大鼠骨骼肌在静息状态下的BCAA分解代谢能力，而且还增强了运动过程中的分解代谢能力。此外，3周的冷暴露显着抑制了体重增加。冷暴露3周的运动训练改善了体重增长的停滞。